RP-HPLC测定清眩丸中欧前胡素的含量  

Determination of Imperatorin in Qingxuan Wan by RP-HPLC

作　　者：谭生建 狄海涛 卢嘉琪[1,2] 付秀娟 常良才[1,2] 

机构地区：[1]国防科工委药检所 [2]中国人民解放军第二炮兵药检所

出　　处：《药物分析杂志》1999年第3期177-178,共2页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的：建立反相高效液相色谱测定清眩丸中欧前胡素含量的方法。方法：采用乙醇做溶剂，水浴加热回流提取。ＬＵＮＡ－Ｃ１８色谱柱分离测定，流动相为乙腈－水（５３∶４７），流速１ｍＬ·ｍｉｎ－１，检测波长３００ｎｍ。结果：欧前胡素与其相邻峰的分离度是１５，理论板数按欧前胡素峰计算是１６１５５，欧前胡素对照品线性范围是１４６２～４８７５μｇ·ｍＬ－１，加样平均回收率９８０％。结论：用本文方法测定清眩丸中欧前胡素的含量，操作简便，结果准确。Objective: A reversed phase method was developed for the determination of imperatorin in Qingxuan Wan. Method: Sample was refluxed with ethanol in a water bath . Chromatographic conditions included a LUNA C18. column,mobile phase, acetonitrile-water (5347), flow rate 1 mLmin-1; SPD6AV uv-vis spectrophotometric detector at wavelength of 300 nm . Results: The number of theoretical plates calculated by imperatorin peak was 16155, R=15. The standard curve was linear in the concentration range of 14624875 gmL-1.The average recovery was 980%. Conclusion: The method was sensitive, rapid and accurate.

关 键 词：清眩丸 欧前胡素 含量 测定 RP-HPLC 

分 类 号：R286.0[医药卫生—中药学]