蜂病康合剂的质量标准研究  

Study on Quality Standard For Fengbingkang Mistura

作　　者：贾永艳[1] 李九席[1] 田效志[1] 卜水[1] 周红敏[1] 徐艳华[1] 

机构地区：[1]河南中医学院,河南郑州450008

出　　处：《中医学报》2010年第5期909-911,共3页Acta Chinese Medicine

基　　金：河南省重点科技攻关计划(编号:072102130006)

摘　　要：目的:建立蜂病康合剂的质量控制方法。方法:以薄层色谱(TLC)法对大青叶、黄芩进行鉴别,用高效液相色谱(HPLC)法测定合剂中黄芩苷含量,以甲醇-水-醋酸(50:50:1)为流动相;检测波长274nm;理论塔板数以黄芩苷计不低于1500。结果:黄芩苷线性范围0.36-1.80μg,平均回收率为98.67%,RSD=0.90%。结论:建立的质量标准稳定、可行、重现性好,为该产品的质量控制奠定了基础。Objective：To build the quality control standard method of Fengbingkang mistura.Methods：TLC was used to identify Isatis indigotica Fort,baical skullcap root.HPLC was used to determine the content of baicalin.Using Methanol-Water-ethanoic acid（50：50：1） as the mobile phase,the flow rate was 0.5 mL/min,the detective wave length was 274 nm.Results：For baicalin the linear range was 0.36-1.80 μg and the average recovery was 98.67% with RSD = 0.90%.Conclusion：This method could be used for the quantitative analysis of Fengbingkang mistura.

关 键 词：蜂病康合剂 黄芩苷 薄层色谱法 高效液相色谱法 

分 类 号：R283.6[医药卫生—中药学]