云南哈尼族CSF_1PO,TPOX和THO_1基因座多态性分析  

Distribution of CSF 1PO, TPOX and THO 1 Loci in Hani Ethnic Group in Yunnan Province

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作  者:李建京[1] 杨燕 邹苹 邹浪萍 李德林[1] 申滨[1] 

机构地区:[1]昆明医学院,昆明650031 [2]云南省人民医院,昆明650032 [3]云南省法院司法鉴定科学技术研究所,昆明650021

出  处:《昆明医学院学报》1999年第2期35-38,50,共5页Journal of Kunming Medical College

基  金:国家自然科学基金

摘  要:应用3 个 S T R 基因座在同一反应体系中进行互不干扰的多重 P C R, 采用高分辨力的聚丙烯酰胺凝胶电泳分离、银染法显影技术, 对云南哈尼族的 C S F1 P O, T P O X 和 T H O1 等3 个基因座等位基因的基因频率进行调查, C S F1 P O 基因座, 观察到7 个等位基因, 17 个基因型; T P O X 基因座, 观察到6 个等位基因,13 个基因型; T H O1 基因座, 观察到6 个等位基因, 19 个基因型. 获得了满意的结果, 显示了广阔的应用前景.The application of short tandem repeats ( STR) polymorphisms has stimulated progress in the elaboration of linkage maps, forensic identification and paternity determination, characterization of disease genes, simplification and precision of DNA typing. DNA extraction from blood and multiplex amplification of CSF 1PO, TPOX and THO 1 were carried out by using denaturing polyacrylamide gel electrophoresis and silver stain. The distribution of allele frequencies of CSF 1PO, TPOX and THO 1 loci in Hani ethnic group living in Yunnan province of China was investigated. 7 alleles and 17 genotypes of CSF 1PO locus were observed. In the locus TPOX, 6 alleles and 13 genotypes were observed. In the THO 1 locus, 6 alleles and 19 genotypes were observed.

关 键 词:CSF1PO TPOX 基因多态性 序列分析 THO1 遗传 

分 类 号:Q346.5[生物学—遗传学]

 

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