紫外分光光度法测定海南木莲叶·枝中总三萜酸的含量  

Ultraviolet Spectrophotometric Determination of Tritepenoidic Acid in the Leaves and Stems of Manglietia hainanensis

作　　者：鲍长余[1] 刘冰晶[1] 毕和平[1,2] 陈光英[1,2] 范超君[1] 

机构地区：[1]海南师范大学化学与化工学院,海南海口571158 [2]海南省热带药用植物化学重点实验室,海南海口571158

出　　处：《安徽农业科学》2010年第23期12460-12461,12466,共3页Journal of Anhui Agricultural Sciences

基　　金：海南省教育厅资助项目(HjKi2008-27);海南省热带药用植物重点实验室资助项目

摘　　要：[目的]测定海南木莲叶和枝中总三萜酸的含量。[方法]以熊果酸为对照品,以质量分数5%香草醛-冰醋酸和高氯酸系统为显色剂,采用紫外分光光度法在546nm波长处测定样品的吸光度。[结果]熊果酸对照品在0.2～1.6μg/ml时呈良好线性关系,回归方程为A=0.1548C+0.0286,r=0.9993。海南木莲叶和枝中总三萜酸的含量分别为1.95%和0.47%。[结论]该方法操作简便,结果稳定,重复性好,易于推广。[Objective] The aim was to determine the content of the tritepenoidic acid in the leaves and the stems of Manglietia hainanensis. [Methed] Under the experimental conditions including ursolic acid as the reference,5% vanillin-acetic acid and perchloric acid as color system,the content of triterpenoidic acid in the leaves and stems of Manglietia hainanensis was determined by ultraviolet spectrophotometric method at 546 nm. [Result] The calibration curve was linear（r=0.999 3）in the range of 0.2-1.6 μg /ml,and the curve was A=0.154 8 C ＋0.028 6,the content was 1.95% and 0.47%. [Conclusion] The result was stable,and had good repeatability. The method operated conveniently and spread easily.

关 键 词：海南木莲 三萜酸 紫外分光光度法 

分 类 号：R284.2[医药卫生—中药学]