烟酰胺在玻碳及聚吡咯修饰电极上的电化学行为  

ELECTROCHEMICAL BEHAVIOR OF NICOTINAMIDE ON GLASSCARBON ELECTRODE AND POLYPYRROLE MODIFIED ELECTRODE

作　　者：程发良[1,2,3,4] 洪茜[1,2,3,4] 莫金垣[1,2,3,4] 戴晓云 

机构地区：[1]东莞理工学院应用化学系 [2]东莞市莞城医院 [3]中山大学化学系 [4]香港科技大学生化系

出　　处：《药学学报》1999年第6期463-466,共4页Acta Pharmaceutica Sinica

摘　　要：目的：研究烟酰胺在玻碳电极及聚吡咯电极上的电化学行为，有助于了解其在人体内的代谢情况。方法：在不同酸度的支持电解质溶液中，分别研究了烟酰胺在玻碳电极和聚吡咯修饰电极上的电化学信息，测试了不同电位范围内烟酰胺的循环伏安电化学信号和电化学反应产物的红外、紫外光谱。结果：在玻碳电极表面，烟酰胺可被分两步电化学还原为二氢吡啶和四氢吡啶结构。四氢吡啶结构的电化学还原产物有电活性，可被分步氧化为环内共轭双键结构和烟酰胺。结论：支持电解质的酸度对氧化还原峰的电流影响较大；聚吡咯（ＰＰｙ）膜对烟酰胺的还原反应有电化学催化作用。提出了烟酰胺电化学反应的机理。AIM: To study the electrochemical behavior of nicotinamide to comprehend its metabolic process in human body. METHODS: At different pH of the supporting electrolyte, the electrochemical behavior of nicotinamide on glasscarbon electrode(GCE) and polypyrrole(PPy) modified GCE was studied with cyclic voltammetry. The electric reaction products of nicotinamide were identified by IR and UV spectrometry. RESULTS: PPy can catalyze the reduction of nicotinamide. The reduction peak potential shifts positively about 150 mV compared to GCE. The electrocatalytic reduction current of nicotinamide on PPy modified GCE is in direct proportion to the 05 order differential of nicotinamide concentration in the range of 1010-21010-6 molL-1. CONCLUSION: The acidity of the supporting electrolyte affect the current of oxidoreduction peak. The PPy film can catalyze the reduction reaction of nicotinamide.

关 键 词：烟酰胺 聚吡咯化学修饰 电极 玻碳 辅酶 

分 类 号：Q552.03[生物学—生物化学]