高效液相色谱-间接光度检测法测定尿中克林霉素浓度  

Determination of clindamycin in urine by high performance liquid chromatographicindirectphotometry

作　　者：梁竹[1] 袁成[1] 焦素云[1] 贡瑞生[1] 

机构地区：[1]中国人民解放军济南军区总医院

出　　处：《中国医院药学杂志》1999年第6期333-335,共3页Chinese Journal of Hospital Pharmacy

摘　　要：目的：建立一种直接测定尿液中克林霉素高效液相色谱间接光度检测法。方法：在流动相中加入具有紫外检测响应的检测剂烟酰胺，用紫外检测器直接测定含量。Ｃ１８固定相，流动相为甲醇乙腈水（２５∶１５∶５５），内含烟酰胺０．５ｍｍｏｌ·Ｌ－１，庚烷磺酸钠５ｍｍｏｌ·Ｌ－１和磷酸０．０５ｍｍｏｌ·Ｌ－１。林可霉素为内标。检测灵敏度为０．０１Ａｕｆｓ，检测波长２６８ｎｍ。结果：平均加样回收率９３．５％±５．１％，ＲＳＤ为５．４％。日内和日间ＲＳＤ均＜７％。测定了３例肌内注射克林霉素患者的尿样品。OBJECTIVE :To establish an high performance liquid chromatographicindirect photoetry(HPLCIP)for determining clindamycin in urine. METHODS :The conditions in the method were with a spherisorb C18 column,the mobile phase of methyl alcoholacetonitrilewater(251555) containing nicotinamide(0.5 mmolL-1),sodium 1heptanesulfonate(5 mmolL-1)and phosphoric acid (0.05 mmolL-1)and the sensitivity of 0.01Aufs. The detection wavelength was fixed at 268 nm. RESULTS :The average recovery for clindamycin was 93.5%5.1%(RSD=5.4%) and the coefficient of variation was less than 7% for withinday and dayto day tests,respectively.The clindamycin concentration of 3 patients was determinde. CONCLUSIONS :The method is convenient,rapid,accurate and reproducible.

关 键 词：克林霉素 尿药浓度 高效液相色谱法 间接光度 

分 类 号：R978.15[医药卫生—药品] R969.1[医药卫生—药学]