高效液相色谱法测定盐酸苯乙双胍片的含量  

Determination of phenformin hydrochloride in tablets by HPLC

作　　者：邢玉仁 马洁 

机构地区：[1]山东省医药工业研究所

出　　处：《中国医院药学杂志》1999年第6期339-340,共2页Chinese Journal of Hospital Pharmacy

摘　　要：目的：建立了高效液相色谱法测定盐酸苯乙双胍片的含量。方法：采用反相Ｃ１８色谱柱，以甲醇醋酸盐缓冲液（ｐＨ４．２）（５５∶４５）为流动相，以二羟丙茶碱为内标物，检测波长为２３４ｎｍ，以峰面积计算。结果：盐酸苯乙双胍在８～５６μｇ·ｍｌ－１的浓度范围内呈良好线性（ｒ＝０．９９９４），平均回收率为９９．９％，ＲＳＤ为０．８４％。结论：本法可用于该片剂的测定，操作简便，结果准确。OBJECTIVE :HPLC method was established for determintation of phenformin hydrochloride tables. METHODS :The chromatographic conditions C18 column,mobile phase of methanolacetate buffer(pH4.2)(5545) and 234 nm for detection intermal standard was diprophylline.RESULTS :There was a good linear relationship with the concentration range of 856 gml-1 of phenformin hydrochloride(r=0.999 4),the average recovery was 99.9%,the relative standard deviation was 0.84%. CONCLUSIONS :The method is selective,simple and accurate.

关 键 词：高效液相色谱法 盐酸苯乙双胍 片剂 

分 类 号：R978.15[医药卫生—药品] R927.2[医药卫生—药学]