胰岛素样生长因子-I对糖尿病大鼠心脏功能的影响  

Effects of insulin like growth factor I on cardiac function of diabetic rats

作　　者：叶红英[1] 俞茂华[1] 游利[1] 杨秀芳[1] 朱禧星[1] 

机构地区：[1]上海医科大学附属华山医院糖尿病研究室

出　　处：《中华内分泌代谢杂志》1999年第3期138-141,共4页Chinese Journal of Endocrinology and Metabolism

摘　　要：目的观察人重组胰岛素样生长因子Ｉ（ｒｈＩＧＦＩ）对ＳＴＺ诱发的糖尿病大鼠心脏机械功能的作用，并和牛初乳短链胰岛素样生长因子Ｉ（ＢＣｔＩＧＦＩ）相比较。方法ＳＴＺ５５ｍｇ／ｋｇ空腹腹腔注射（ＩＰ）制备糖尿病大鼠模型，随机分为四组：ＤＭＣ组，生理盐水０．１ｍｌ／ｄＩＰ；ＤＭＬ组，ＢＣｔＩＧＦＩ２５０ｎｇ·ｋｇ－１·ｄ－１ＩＰ；ＤＭＨ组，ＢＣｔＩＧＦＩ７５０ｎｇ·ｋｇ－１·ｄ－１ＩＰ；ＤＭｒｈ组，ｒｈＩＧＦＩ７５０ｎｇ·ｋｇ－１·ｄ－１ＩＰ；并设正常对照组，每组５只。实验持续６周。心功能用室内导管法测定，观察指标有：室内压最大值（Ｐｅａｋ）、舒张末期室内压（ＥＤＰ）、室内压上升段最大变化率（ｄｐ／ｄｔ）、室内压下降段最大变化率（－ｄｐ／ｄｔ）。同时测定尾尖血血糖（ＢＧ）、血清果糖胺（ＦＭＮ）、甘油三酯（ＴＧ）、胆固醇（Ｃｈ）。结果与正常大鼠相比，ＤＭＣ组血清ＦＭＮ、ＴＧ明显升高，同时Ｐｅａｋ降低４１．４％，ｄｐ／ｄｔ、ｄｐ／ｄｔ绝对值分别降低４０．３％、５８．５％，ＥＤＰ升高〔（２．００±０．６２）ｍｍＨｇｖｓ（－０．４４±０．２６）ｍｍＨｇ，Ｐ＜０．０１〕。ＤＭＬ、ＤＭＨ、Ｄ?Objective To investigate the effects of recombinant human insulin like growth factor I (rhIGF I) on cardiac function of STZ induced diabetic rats and compare with these of bovine colostrum truncated IGF I (BC tIGF I). Methods Diabetes was induced in male SD rats (180～220g) by intraperitoneal injection (IP) of STZ at a dose of 55mg/kg and animals were divided into four groups: DM C group (n=5), treated with NS 0.1ml/d IP; DM L group (n=5), treated with BC tIGF I 250ng·kg -1 ·d -1 , IP; DM H group (n=5), treated with BC tIGF I 750ng·kg -1 ·d -1 , IP;DM rh group (n=5), treated with rhIGF I 750ng·kg -1 ·d -1 , IP. Therapy lasted 6 weeks. Cardiac functions including heart rate (HR), LV systolic pressure (Peak), LV end diastolic pressure (EDP), rate of LV contraction (dp/dt) and rate of LV relaxation (-dp/dt) were investigated after a catheter was inserted into carotid artery, then into left ventricular. The plasma was separated for analysis of BG、FMN, Ch and TG. Results Compared with normal group, DM C group had a markedly increased FMN and TG, a 41.4% decrease in Peak, a 40.3% decrease in dp/dt, a 58.5% decrease in -dp/dt and an increased EDP (2.00±0.62)mmHg vs (-0.44±0.26)mmHg, P<0.01 , showing impaired cardiac function. The FMN and TG of DM L, DM H and DM rh were obviously lower than those of DM C, but still higher than those of normal group. The indices of myocardial function of DM L and DM rh were similar to those of normal group (P>0.05).Conclusion IGF I improves metabolism and has a beneficial effect on myocardial functions of STZ induced diabetic rats.

关 键 词：糖尿病 心脏功能 RHIGF-I 大鼠 

分 类 号：R587.2[医药卫生—内分泌] R541.02[医药卫生—内科学]