紫杉醇对血管外膜成纤维细胞基质金属蛋白酶-2和α-平滑肌肌动蛋白的影响  

Effect of paclitaxel on the expression of vascular's fibroblast of matrix metalloproteinases-2 and α-smooth muscle actin

作　　者：沈凯[1] 刘芳洁[1] 袁国裕[1] 顾磊科[1] 王红娜[1] 陈国雄[1] 方波[1] 

机构地区：[1]舟山市人民医院心内科,浙江舟山316000

出　　处：《中国临床药理学杂志》2010年第9期665-667,共3页The Chinese Journal of Clinical Pharmacology

基　　金：浙江省医药卫生科学研究基金资助项目(2007A198);浙江省中医药科技计划基金资金项目(2007CA129)

摘　　要：目的研究紫杉醇(抗癌药)对成纤维细胞基质金属蛋白酶-2(MMP-2)和α-平滑肌肌动蛋白(α-SM-actin)的影响。方法选取雄性SD大鼠,用贴壁法培养胸主动脉外膜成纤维细胞(AF),用RT-PCR方法检测α-SM-actin和MMP-2。结果成纤维细胞的MMP-2的表达量,紫杉醇组较对照组也明显降低(P<0.01);α-SM-actin的表达量,紫杉醇组较对照组明显降低(P<0.05),表明紫杉醇能抑制AF的MMP-2和α-SM-actin的表达,抑制AF的表型转化,从而抑制血管外膜的迁移。结论紫杉醇可防治冠状动脉介入术后再狭窄。Objective To investigate the effect of paclitaxel on the ex- pression of matrix metalloproteinases - 2 （ MMP - 2 ） and α - smooth muscle aetin （ α- SM - actin） of the fibroblast. Methods Adventitial fibroblast （AF） were prepared from rat aortas using explant technique and cultured for passages, the expressions of MMP - 2 and α - SM - actin were determined by RT - PCR. Results The expression of MMP - 2 in the paclitaxel group is significantly lower than that in the control group （P 〈 O. 01 ）. The expression of α - SM - aetin in the paclitaxel group is signif- icantly lower than that in the control group（ P 〈 0. 01 ）. Paclitaxel can in- hibit the expression of MMP - 2 and α - SM - actin of the fibroblast, ef- fectively inhibiting the phenotype transformation of AF, thus inhibiting the multiplication and migration of vascular adventitia. Conclusion Paclita- xel can prevent restenosis after percutaneous coronary intervention.

关 键 词：紫杉醇 血管外膜 冠状动脉介入术后再狭窄 Α-平滑肌肌动蛋白 基质金属蛋白酶-2 

分 类 号：R979.1[医药卫生—药品] R965.1[医药卫生—药学]