丛枝菌根真菌光自养培养体系的建立  

Establishment of a Photoautotrophic Culture System for the in vitro Mycorrhization of Medicago truncatula Plantlets

作　　者：陈浩[1] 肖翔[2] 王军[1] 吴李君[1] 

机构地区：[1]中国科学院离子束生物工程学重点实验室,安徽合肥230031 [2]中国科学技术大学化学与材料科学学院,安徽合肥230026

出　　处：《安徽农业科学》2010年第25期13605-13606,共2页Journal of Anhui Agricultural Sciences

基　　金：中国科学院合肥物质科学研究院院长基金项目

摘　　要：[目的]建立以蒺藜苜蓿(Medicago truncatula)为寄主的丛枝菌根真菌光自养培养体系。[方法]以丛枝菌根真菌(Glomus etunica-tum)为试验材料,以豆科模式植物——蒺藜苜蓿为寄主,建立植物根系在严格的培养条件下与AMF的共生关系,而植物的地上部分可以进行正常的光合作用,并对孢子产量、根的侵染情况以及后代孢子的活力进行了评估。[结果]孢子能正常侵染植物并产生大量新生孢子,新生孢子在相同条件下可以继代培养。[结论]本研究为在光自养条件下多方位研究菌根真菌提供了实验平台。[Objective]The aim is to establish an photoautotrophic culture system for the in vitro mycorrhization of Medicago truncatula plantlets.[Method]With the arbuscular mycorrhizal fungi Glomus etunicatum as the experiment material and Leguminous model plant-Medicago truncatula as the host,the Photoautotrophic culture system,under which a symbiosis between plant roots and AMF is established under in vitro conditions while shoots developed under open air conditions,is established.Moreover,the spore yield,root infection as well as activity of spore progeny are evaluated.[Result]Spore could normally infect plants and produce a large number of new spores,which could be sub-cultured under the same conditions.[Conclusion]The research provides an experiment platform for studying mycorrhizal fungi under photoautotrophic conditions from various aspects.

关 键 词：光自养培养 蒺藜苜蓿 丛枝菌根真菌 

分 类 号：S311[农业科学—作物栽培与耕作技术]