果蝇唾腺染色体的制作技术研究  

Observation Technique on Drosophila Salivary Gland Chromosome

作　　者：任莉萍[1] 聂永青[1] 许树成[1] 

机构地区：[1]阜阳师范学院生命科学学院,安徽阜阳236032

出　　处：《安徽农业科学》2010年第25期13609-13610,共2页Journal of Anhui Agricultural Sciences

基　　金：阜阳师范学院自然科学项目(2008LQ08);阜阳师范学院质量工程项目(2009SYZX02)

摘　　要：[目的]为果蝇唾腺染色体试验教学及相关研究提供成熟的制作技术。[方法]以野生型果蝇三龄幼虫为试验材料,通过三龄幼虫培养、唾腺剥离、压片、解离、染色等完整的制片过程探索染色体制片规律。[结果]利用镊子柄部进行压片效果很好,染色体伸展充分。通过比较4种染色试剂(苏木精、醋酸洋红、卡宝品红和改良苯酚品红)的染色效果选出的最佳试剂为改良苯酚品红。[结论]该研究为果蝇唾腺染色体的制作提供了成熟的技术参考。[Objective]The aim of this study was to provide mature manual technique for experiment teaching of Drosphila salivary gland chromosome and other related research.[Method]The three-days larvas of wild Drosphila were took as experiment materials.The making rule of temporary chromosome specimen was explored throughout the complete course.[Result]By comparison,the staining effect of modified phenol fuchsion was much better than other conventional methods.[Conclusion]This study has provided the mature technical reference for the making of Drosphila salivary gland chromosome.

关 键 词：果蝇 唾腺染色体 染色试剂 

分 类 号：Q343.2[生物学—遗传学]