大孔吸附树脂对金铁锁醇提液的除杂工艺研究  

Study on Purification Technology of Macroporous Adsorptive Resin on Alcohol Extracts of Psammosilene tunicoides W.C.Wu et C.Y.Wu

作　　者：梁娟[1] 曹国琼[1] 贾宪生[1] 胡成刚[1] 

机构地区：[1]贵阳中医学院药学系,贵州贵阳550002

出　　处：《安徽农业科学》2010年第25期13682-13683,13708,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]优化金铁锁醇提液的除杂工艺。[方法]以金铁锁药材为原料,采用75%乙醇提取其中的皂苷类化合物,并采用D101大孔吸附树脂对提取液进行除杂,通过正交试验研究大孔树脂用量、洗脱剂(乙醇)浓度及用量对总皂苷得量的影响。[结果]各因素对总皂苷得量的影响依次为:大孔树脂用量>乙醇浓度>洗脱剂用量;最佳除杂工艺为:D101大孔吸附树脂用量10g,以200ml60%的乙醇为洗脱剂进行洗脱。[结论]该研究确定了大孔吸附树脂对金铁锁醇提液的最佳除杂工艺。[Objective]The study was to optimize the purification technology of alcohol extracts of Psammosilene tunicoides W.C.Wu et C.Y.Wu.[Method]The P.tunicoides herbs were taken as the raw materials.The steroid saponins in them were extracted by 75% ethanol,and the extract was purified by D101 macroporous adsorptive resin.The effects of macroporous adsorptive resin dosage,eluent （ethanol） concns.and dosage on yield rate of total saponins were studied through orthogonal test.[Results]The effect of factors on yield rate of total saponins in order was：macroporous resin dosage 〉ethanol concn.〉 eluent dosage.The optimum purification technology was D101 macroporous adsorptive resin dosage of 10 g,eluent concns of 60% and eluent dosage of 200 ml.[Conclusion]This study identified the optimum purification technology of macroporous adsorptive resin on alcohol extracts of Psammosilene tunicoides.

关 键 词：金铁锁 大孔树脂 正交试验 

分 类 号：S681.5[农业科学—观赏园艺]