反相高效液相色谱-二极管矩阵检测法测定小蔓长春花中长春胺  

RP-HPLC-PDA Determination of Vincamine in Vinca minor L.

作　　者：侯恩太[1] 李多伟[1] 倪士峰[1] 王英娟[1] 韩丽娟[1] 王乐[1] 

机构地区：[1]西北大学生命科学学院,陕西西安710069

出　　处：《安徽农业科学》2010年第25期13696-13698,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(30970308);中国科学院"西部之光"人才培养计划(08DF01);陕西省教育厅专项(09J-K775)

摘　　要：[目的]建立测定小蔓长春花中长春胺含量的方法,测定不同年龄不同采收季节小蔓长春花中长春胺含量。[方法]采用Inert-silODS-3色谱柱(4.6mm×150mm,5μm),以Waters-2996PDA为检测器,外标法定量。流动相为甲醇-1%二乙胺(磷酸调pH值至7.5,体积比65:35),流速为1.0ml/min,检测波长为279.5nm。[结果]长春胺在0.05～0.50mg/ml范围内与峰面积线性关系良好(R2=0.997),平均加样回收率为100.71%(n=5),RSD=2.23%。长春胺峰保留时间为10.8min。[结论]该检测方法可用于小蔓长春花药材的质量监控。不同生长年龄不同采收季节小蔓长春花中长春胺含量有明显差异。[Objective]It aims to establish a method to determine the vincamine content in Vinca minor L.of different ages in different harvesting season.[Method]Inertsil ODS-3 column （4.6 mm×150 mm,5 μm） and Waters-2996 PDA detector is used for qualitative and quantitative analysis by external standard method.The mobile phase consisted of methanol-1% diethylamine （pH value of phosphorus and acid reduces to 7.5,the ratio of p to acid is 65：35）,flow rate is 1 ml/min.The detection wave length was 279.5 nm.[Results]Within 0.05-0.50 mg/ml,vincamine has a good linearity with peak area （R2=0.997）.The average recovery is 100.71%（n=5） with RSD=2.23%.The retention time of vincamine is 10.8 min.[Conclusion]The method could be used for quantitative determination of Vinca minor L..The vincamine content in different ages of Vinca minor L.at different harvesting seasons differs significantly.

关 键 词：小蔓长春花 长春胺 反相高效液相色谱 二极管阵列检测 

分 类 号：S685.99[农业科学—观赏园艺]