检测人巨细胞病毒晚期mRNA在诊断子宫内活动性感染的价值  

Diagnostic Value of Human Cytomegalovirus LatemRNA Detection for Intrauterine Active Human Cytomegalovirus Infection

作　　者：姜宏[1,2] 闻良珍[1,2] 凌霞珍[1,2] 陈素华 

机构地区：[1]同济医科大学附属同济医院妇产科 [2]湖南医科大学生殖工程研究室

出　　处：《中华妇产科杂志》1999年第6期345-347,共3页Chinese Journal of Obstetrics and Gynecology

基　　金：国家"九五"重点攻关项目资助

摘　　要：目的探讨人巨细胞病毒（ＨＣＭＶ）晚期ｍＲＮＡ检测在宫内活动性感染中的应用价值。方法采用逆转录聚合酶链反应（ＲＴＰＣＲ）法对４２例ＨＣＭＶＩｇＭ阳性孕妇外周血及部分胎儿附属物进行ＨＣＭＶ晚期ｍＲＮＡ检测。结果４２例ＨＣＭＶＩｇＭ阳性孕妇中，检出晚期ｍＲＮＡ２３例，两者符合率为５４．７％。对其中１３例晚期ｍＲＮＡ阳性孕妇胎儿附属物进行检测，７例阳性，母婴传播率为５３．８％，而１２例晚期ｍＲＮＡ阴性，ＨＣＭＶＤＮＡ阳性孕妇胎儿附属物中仅１例阳性，母婴传播率为８．３％，两者比较，差异有非常显著性，（Ｐ＜０．０１）。结论ＨＣＭＶＩｇＭ的阳性结果并不能完全准确地显示受检时ＨＣＭＶ的活动状态；ＨＣＭＶ的活动状态与母婴传播情况密切相关，晚期ｍＲＮＡ的检测不仅能够显示ＨＣＭＶ活动性感染时的宫内传播情况，而且可以了解受检时胚胎或胎儿组织内ＨＣＭＶ的活动状态。Objective To study diagnostic value of latemRNA detection for intrauterine active human cytomegalovirus (HCMV) infection. Methods The HCMV latemRNA in peripheral blood of the pregnant women with HCMVIgM positive and their fetal appendages (such as chorionic villi,amniotic fluid,umbilical blood and placenta) were detected by reverse transcription polymerase chain reaction(RTPCR). Results The latemRNA was detected in 23 out of 42 HCMVIgM positive pregnant cases, with the 54.3% of corresponding rate between the results of HCMVIgM and HCMV latemRNA.7 samples out of 13 fetal appendages from mRNA positive mothers were latemRNA positive, while in 12 mothers with latemRNA negative group only one fetal sample was latemRNA positive, Between two groups there was significant difference in motherfetus transmission rate. Conclusions The positive result of HCMVIgM can not accurately reflect HCMV activity at the time being detected, which is closely related to the motherfetus transmission rate. As a symbol of active HCMV infection,latemRNA can not only reflect the motherfetus transmission rate during active HCMV infection correctly,but also provide some information about activity of the HCMV in fetal tissue, It also has a great value in prediction for prognosis of infectious fetuses.

关 键 词：巨细胞病毒 MRNA 子宫内感染 孕妇 

分 类 号：R714.251[医药卫生—妇产科学] R373.9[医药卫生—临床医学]