抗虫活性菌株SIPI-9764的产物研究  

Studies on the metabolites of actinomyces SIPI9764

作　　者：孟晓峰[1] 张礼萍[1] 龚炳永[1] 

机构地区：[1]上海医药工业研究院新药研究室

出　　处：《中国抗生素杂志》1999年第3期173-174,225,共3页Chinese Journal of Antibiotics

摘　　要：放线菌ＳＩＰＩ－９７６４的发酵液经溶媒萃取、硅胶柱层析和结晶等，分离出２个异黄酮类化合物，命名为ＳＩＰＩ－９７６４－Ⅰ和－Ⅱ。通过对其理化性质和ＵＶ、ＩＲ、ＭＳ、ＮＭＲ图谱的分析，确定化合物ＳＩＰＩ－９７６４－Ⅰ和－Ⅱ分别为４′，５，７－Ｔｒｉｈｙｄｏｘｙｉｓｏｆｌａｖｏｎｅ和４′，７－Ｄｉｈｙｄｒｏｘｙｉｓｏｆｌａｖｏｎｅ，与已知的金雀异黄素（ｇｅｎｉｓｔｅｉｎ）和黄豆苷元（Ｄａｉｄｚｅｉｎ）相一致。两个化合物显示出一定的Ｎａ＋／Ｋ＋ＡＴＰａｓｅ抑制活性和抗虫活性，其在１ｍｇ／ｍｌ的浓度对线虫的击倒率分别为７３％和６４％。From the broth of actinomyces SIPI9764, two isoflavones were isolated by solvent extraction, silica gel chromatograbhy and crystallization, which were named as SIPI9764 and . The structures of SIPI9764 and were elucidated by means of physicochemical properties, UV, IR, MS and NMR spectra. They were 4,5,7Trihydroxyisoflavone and 4,7Dihydroxyisoflavone which were identical with Genistein and Daidzein. These tow isoflavones showed Na+/K+ ATPase inhibitory activities and insecticide activities. SIPI9764 and had 73% and 64% insect killing effect at concentration of 1mg/ml respectively.

关 键 词：异黄酮 抗虫化合物 放线菌 SIPI-9764 药物筛选 

分 类 号：R978.5[医药卫生—药品] R965.1[医药卫生—药学]