2,4-D对盐藻生物量与物质积累的影响  

Effects of 2,4-D on the biomass and nutrient accunulation of Dunaliella salina

作　　者：孟春晓[1] 高政权[1] 王依涛[1] 叶乃好[2] 

机构地区：[1]山东理工大学生命科学院,山东淄博255049 [2]中国水产科学院黄海水产研究所,山东青岛277071

出　　处：《黑龙江畜牧兽医》2010年第9期1-3,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(40706050;4070648;30700619);"十一五"国家科技支撑计划项目(2006BAD01A13);国家基因改良专项基金项目(2009ZX08009-019B);山东省自然科学基金项目(2009ZRA2075);青岛市科技计划项目(09-2-5-8-hy)

摘　　要：为了研究2,4-D对杜氏盐藻生物量和物质积累的影响,试验采用向对数生长期的杜氏盐藻(Dunaliella salina)中分别添加浓度为0.01,0.05,0.1,0.5,1,5,10,25,50 mg/L植物激素2,4-D对其进行诱导培养,周期性收集藻细胞,对各阶段藻细胞中细胞密度、β-胡萝卜素、叶绿素、总可溶性蛋白含量进行检测和比较。结果表明:在1个月的诱导期内,用0.1 mg/L 2,4-D处理不但能显著加快藻细胞的分裂速度,而且比对照组的生物量提高了22%,β-胡萝卜素的产量增加了7.8%;但该处理的叶绿素含量、总可溶性蛋白含量与其他处理组及对照组都没有明显的区别。To study the effect of extraneous 2,4-D on the growth and biochemical composition of Dunaliella salina,different concentrations of 2,4-D were added into the vegetative green cells.In this stage,cell density,β-carotene,chlorophyll and total soluble proteins contents were determined respectively.The results showed that cell density and β-carotene production of 0.1 mg/L 2,4-D treatment were 22% and 7.8% higher than those of the control.Chlorophyll and total soluble proteins contents of this treatment had no evident difference from those of other treatments and the control.

关 键 词：杜氏盐藻 2 4-二氯苯氧乙酸(2 4-D) 细胞密度 β-胡萝卜素 叶绿素 总可溶性蛋白 

分 类 号：S917.3[农业科学—水产科学]