钝顶螺旋藻粗多糖抗氧化作用的研究  

The anti-oxidative effect of Spirulina (Arthrospira) patensis polysaccharide in vitro

作　　者：赵飞艳[1] 敖长金[1] 杜玲[1,2] 曹平[1] 

机构地区：[1]内蒙古农业大学动物科学与医学学院,内蒙古呼和浩特010018 [2]内蒙古师范大学生命科学与技术学院,内蒙古呼和浩特010022

出　　处：《黑龙江畜牧兽医》2010年第9期14-16,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(30460104);内蒙古自然科学基金(200308020310)

摘　　要：为了研究钝顶螺旋藻多糖的抗氧化作用,试验用羟自由基(.OH)测定试剂盒和超氧阴离子自由基(O2.-)测定试剂盒测定了钝顶螺旋藻多糖对自由基的体外清除能力;同时还用总超氧化物歧化酶(T-SOD)测定试剂盒和总抗氧化能力(T-AOC)测定试剂盒测定了钝顶螺旋藻多糖在体内的抗氧化能力。结果表明:在体外,螺旋藻多糖对.OH和O2.-均具有明显的清除作用,以1.0 mg/mL和6 mg/mL的作用效果最显著(P<0.05);在体内,钝顶螺旋藻多糖能显著提高小鼠T-SOD和T-AOC水平,其中以625 mg/kg的作用效果最为明显(P<0.05)。说明钝顶螺旋藻多糖能够通过提高抗氧化酶的活性提高机体的抗氧化能力。To explore the antioxidant activity of polysaccharide from Spirulina（Arthrospira） platensis（PSP）,scavenging effects of PSP on active oxygen species ·OH and O2·-were studied by ·OH test box and O2·-test box in vitro,and then the anti-oxidations of PSP were investigated in vivo by T-SOD test box and T-AOC test box.The result showed that PSP could significantly scavenge ·OH and O2·-in vitro.The highest scavenging effects occurred in the 1.0 mg/mL and 6 mg/mL,the scavenging effects were different significantly with different dosage（P0.05）.The activity of T-SOD and T-AOC in mice were increased remarkably,but the effect of 625 mg/kg were the best significant（P0.05）.It concludes that PSP can enhance the anti-oxidation capacity of the body by means of increasing activity of antioxidat enzymes.

关 键 词：钝顶螺旋藻 多糖 羟自由基 超氧阴离子 抗氧化 

分 类 号：S852.4[农业科学—基础兽医学]