绵羊MHC Class Ⅰ区段429P24 BAC克隆的基因筛选与序列分析  

Screening,cloning and sequence analysis of MHC Class Ⅰ section 429P24 BAC clone gene of sheep

作　　者：李刚[1] 杨晓亮[1] 谢晓燕[1] 李峰[1] 陈芳[1] 高剑峰[1] 

机构地区：[1]石河子大学生命科学学院,新疆石河子832003

出　　处：《黑龙江畜牧兽医》2010年第9期17-19,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家科技部国际合作重大项目(2006DFB33750)

摘　　要：为了建立细菌人工染色体(BAC)筛选绵羊cDNA文库的方法,并期望分离到与绵羊疾病有关的基因,试验以429P24 BAC克隆为探针,采用噬菌斑杂交筛选法筛选绵羊cDNA文库,对分离出的cDNA阳性克隆进行测序和计算机序列比较。结果表明:以429P24 BAC克隆为探针筛选出7个阳性克隆,经初步分析可能是与绵羊的免疫和疾病有关的基因。说明以429P24 BAC克隆为探针筛cDNA文库是一种有效的表达序列分离法。To introduce a method to isolate cDNA clones using bacterial artificial chromosome（BAC） as probe for hybridization and try to find some genes related to immune and disease of sheep.Experiment used 429P24 BAC to screen the sheep cDNA library as probe for hybridization.The isolated positive cDNA clones were partially sequenced,then analyzed by computer comparison.The results indicated 7 candidate positive cDNA clones were isolated using 429P24 BAC probes.Further analysis indicated these genes may be related to immune and disease of sheep.Conclusions showed the isolation of cDNA clones using 429P24 BAC probes was effective and practical.The result revealed that these genes may be related to immune and disease of sheep.

关 键 词：32P标记 绵羊 MHC CDNA文库 DNA探针 

分 类 号：S511[农业科学—作物学]