水稻条纹病毒NS3蛋白与水稻3-磷酸甘油醛脱氢酶(GAPDH)间的互作  

Interaction Between Rice Stripe Virus NS3 Protein and Glyceraldehyde-3-Phosphate Dehydrogenase(GAPDH) of Rice

作　　者：肖冬来[1] 贾东升[1] 吴建国[1] 杜振国[1] 谢荔岩[1] 吴祖建[1] 谢联辉[1] 

机构地区：[1]福建农林大学植物病毒研究所/福建省植物病毒学重点实验室,福建福州350002

出　　处：《中国水稻科学》2010年第5期493-496,共4页Chinese Journal of Rice Science

基　　金：国家转基因生物新品种培育重大专项资助项目(2009ZX08009-044B);国家自然科学基金资助项目(30770090);农业部农业公益性行业科研专项资助项目(nyhyzx07-051)

摘　　要：水稻条纹病毒(rice stripe virus,RSV)NS3蛋白为病毒的沉默抑制子。利用酵母双杂交技术,从水稻cDNA文库中筛选出一个与RSV NS3蛋白互作的基因片段。推测该基因的功能是编码水稻3-磷酸甘油醛脱氢酶(glyceraldehyde-3-phosphate dehydrogenase,GAPDH)。双分子荧光互补实验进一步验证了NS3与GAPDH存在互作。瞬时表达实验表明,GAPDH与GFP基因融合蛋白在本氏烟表皮细胞细胞质中大量积累。此外,讨论了GAPDH蛋白在RSV侵染水稻过程中可能的功能。NS3 protein of rice stripe virus （RSV） is an RNA silencing suppressor. Yeast two-hybrid assay was used to screen the rice cDNA library with the NS3 protein as a bait. A novel rice protein interacting with RSV NS3 was obtained. The putative function of the interacting protein was glyceraldehyde-3-phosphate dehydrogenase （GAPDH）. Bimolecular fluorescence complementation （BiFC） also showed that RSV NS3 could interact with the GAPDH. Cellular localization studies showed that GAPDH-GFP fusion protein accumulated predominantly in cytoplasm of tobacco （Nicotiana benthamiana） through transient expression assay. Possible functions of GAPDH during the infection of rice by RSV were discussed.

关 键 词：水稻条纹病毒 蛋白功能 互作 酵母双杂交 双分子荧光互补 

分 类 号：S435.11[农业科学—农业昆虫与害虫防治]