总红细胞破损在滚柱泵体外转流实验中存在的研究  

Investigating the Phenomenon of Total Erythrocyte Destruction During an In Vitro Hemolysis Test by Roller Pump

作　　者：徐世伟[1] 陈如坤[1] 陈芳[1] 胡自强[1] 丁敏君[1] 陆水根[1] 

机构地区：[1]浙江医科大学附属第二医院心胸外科,杭州310009

出　　处：《生物医学工程学杂志》1999年第2期240-242,共3页Journal of Biomedical Engineering

基　　金：浙江省卫生厅科研经费

摘　　要：观察滚柱泵在长时间转流中是否存在“总红细胞破损”现象。用Ｐｏｌｙｓｔａｎ 泵（Ａ 组）和Ｃｏｂｅ 泵（Ｂ组）转流库存１ ｄ ＡＣＤ 血４００ ｍ ｌ，用联苯胺显示法测定游离血红蛋白，用Ｋｏｌｌｅｒ氏法计算溶血指数。血样本为转流前，转中４、６、８、１０、１２、１４、１６ ｈ。结果：两组游离血红蛋白随转流时间延长，逐渐增高。两组溶血指数分别为０．２９６０ ｍ ｇ／Ｌ和０．３９９３ ｍ ｇ／Ｌ，两组比较无显著差异。在滚柱泵长时间体外转流实验中未观察到“总红细胞破损”现象。This study sought to address the question, “will the phenomenon of ‘total erythrocyte destruction’ come about during a long time of in vitro hemolysis test by roller pump”. One day old boving blood was employed in the test by Polystan pump(group A) and Cobe pump (group B).TMB method was used to measure the level of plasma free hemoglobin. The samples of plasma free hemoglobin were taken at the time before pumping and pumping for 4,6,8,10,12,14 and 16 hours. The index of hemolysis was calculated according to Koller's formula. The results showed that the levels of plasma free hemoglobin in the two groups both increased linearly during the puming of blood. The index of hemolysis was 0.2960 mg/L in group A, and 0.3993 mg/L in group B; no statistically significant difference was noted between the two groups. These data indicated that no phenomenon of “total erythrocyte destruction”was observed in this hemolysis test by roller pump.

关 键 词：体外循环 血泵 溶血 总红细胞 体外转流 

分 类 号：R654.1[医药卫生—外科学]