Toll样受体2、4在麻风患者外周血单一核细胞中的表达  

Expressions of Toll-like receptor 2 and 4 in peripheral blood monouclear cells of patients with leprosy

作　　者：罗丽[1] 刘源[1] 张信江[1] 鲁东平[1] 张庆波[1] 王宝仁[2] 刘放鸣 

机构地区：[1]遵义医学院附属医院皮肤科,贵州563003 [2]贵州毕节地区医院皮肤科 [3]贵州毕节地区疾病预防控制中心

出　　处：《中华皮肤科杂志》2010年第9期623-625,共3页Chinese Journal of Dermatology

基　　金：国家自然科学基金（30960350）

摘　　要：目的 探讨Toll样受体2、4 mRNA在麻风患者外周血单一核细胞中的表达.方法 运用SYBR Green荧光定量RT-PCR技术检测麻风现症患者与已痊愈患者各30例外周血单一核细胞中Toll样受体2、4 mRNA的表达水平,并用正常人作对照.结果 Toll样受体2 mRNA在麻风患者外周血单一核细胞中表达明显高于痊愈组和正常人对照组（P＜0.01）.Toll样受体4 mRNA在麻风现症患者外周血单一核细胞中的表达与痊愈组和正常人对照组差异无统计学意义（P＞0.05）.结论 Toll样受体2 mRNA在麻风患者外周血单一核细胞的表达明显升高,提示Toll样受体2 mRNA可能是麻风杆菌的特异识别受体.Objective To study the mRNA expressions of Toll-like receptor （TLR） 2 and 4 in peripheral blood mononuclear cells （PBMCs） of patients with leprosy. Methods SYBR Green fluorescence quantitative RT-PCR was conducted to detect the mRNA expressions of TLR2 and 4 in PBMCs from 30 patients with cured leprosy, 30 patients with active leprosy and 30 normal human controls. Results The expression of TLR2 mRNA in patients with active leprosy was significantly higher than that in those with cured leprosy and in controls （both P ＜ 0.01 ）, but there was no significant difference between the latter two groups （P ＞ 0.05）. The expression of TLR4 mRNA was of no significant difference between patients with active leprosy and those with cured leprosy orthe controls （both P＞ 0.05）. Conclusion The expression of TLR2 mRNA is decreased in PBMCs from leprosy patients, suggesting that TLR2 may be a specific recognition receptor in patients with leprosy.

关 键 词：麻风 TOLL样受体2 TOLL样受体4 单核细胞 

分 类 号：R755[医药卫生—皮肤病学与性病学]