包裹阿霉素超声造影剂的制备及体外抗肿瘤作用研究  

Preparation and Antitumor Effect of Doxorubicin Ultrasound Contrast Agent

作　　者：张子栋[1] 严飞[2] 李翔[2] 靳巧锋[2] 常胜合[1] 郑海荣[3] 

机构地区：[1]郑州大学河南省离子束生物工程重点实验室,河南郑州450052 [2]中国科学院深圳先进技术研究院生物医学与健康工程研究所保罗.C.劳特伯生物成像研究中心,广东深圳518055 [3]中国科学院生物医学信息与健康工程学重点实验室,广东深圳518055

出　　处：《中国医学物理学杂志》2010年第5期2161-2165,共5页Chinese Journal of Medical Physics

基　　金：国家自然科学基金(No.10904094;No.10904095)

摘　　要：目的:研究包裹阿霉素超声造影剂的制备方法,并观察其体外显影及体外抗肿瘤活性。方法:用双乳化法对盐酸阿霉素进行包裹,并测量其粒径;以乳腺癌细胞MD-MBA-231为研究对象,用超声空化效应细胞内给药,以MTT法评价其体外抗肿瘤活性,并与阿霉素裸药进行比较。结果:本实验成功制备了包裹阿霉素的超声造影剂,粒径分布均匀(1μm左右),载药量为3.14%,包封率为35.84%,体外能够显著增强显影效果,作用24小时后,相同浓度抑制率差别不是很大,48小时和72小时后,高浓度给药差异较大。结论:本研究为超声成像与给药一体化研究奠定了基础。Objective：To investigate the preparation and methods of doxorubicin ultrasound contrast agent,and evaluate their ul-trasound imaging and antitumor effect in vitro.Methods：Poly lactic acid（PLA） was employed to fabricate the ultrasound con-trast agent with doxorubicin and air inside by a double emulsion method and measured its particles size.Ultrasound cavitation effect was used to release drugs and to evaluate their anfitumor effect in vitro by MTT assay in MDA-MB-231 cells.Results：This method could produce doxorubicin ultrasound contrast agent with a tight size distribution which average diameter is about 1 μm.The drug loading content was 3.14% and the encapsulation efficiency was 35.84%.It could enhance the ultrasound im-age much more effectively in vitro.The inhibition rate has no obvious difference when treated with the same concentration for 24 hours,however,there is a significant difference when treated with high concentration for 48 hours and 72 hours.Conclu-sions：This experiment will lay a good foundation for further study of ultrasound imaging and drug delivery integration.

关 键 词：阿霉素 抗肿瘤作用 超声造影剂 双乳化法 空化效应 

分 类 号：R445.1[医药卫生—影像医学与核医学] R730.53[医药卫生—诊断学]