高甘油三酯血症中对氧磷酯酶-1活性的研究  

Activity of Paraoxonase-1 in Hypertriglyceridemia

作　　者：郑伟[1,2,3] 周赤燕[1,2] 喻红[1,2] 李小明[1,2] 曹佳[1,2] 胡汉宁[4] 沈丹[5] 

机构地区：[1]武汉大学医学院生物化学与分子生物学系 [2]武汉大学食品与药品评价研究中心,湖北武汉430071 [3]郧阳医学院生物化学教研室,湖北十堰442000 [4]武汉大学中南医院检验科,湖北武汉430071 [5]武汉大学中南医院综合科,湖北武汉430071

出　　处：《武汉大学学报（医学版）》2010年第5期561-564,570,共5页Medical Journal of Wuhan University

基　　金：国家自然科学基金资助项目(编号:30770850);湖北省自然科学基金计划青年杰出人才项目(编号:2007ABB024);武汉大学博士研究生自主科研项目

摘　　要：目的:观察高甘油三酯血症(HTG)状态下高密度脂蛋白(HDL)相关抗氧化酶——对氧磷酯酶-1(PON-1)的活性变化。方法:收集湖北武汉地区40例单纯性HTG患者和65例正常人群的血样,比较血脂分布特征,利用酶水解底物对氧磷的原理测定血浆HDL特征性PON-1的活性;同时以脂蛋白脂肪酶基因敲除(LPL-/-)鼠为HTG动物模型,检测血清中甘油三酯(TG)、总胆固醇(TC)含量和PON-1的活性改变。结果:①HTG组与对照组相比,血浆载脂蛋白(apo)B100水平显著升高(P<0.01),HDL-C、apoA-Ⅰ水平降低(P<0.05);PON-1酶活性显著降低(P<0.001);②HTG合并正常HDL-C组与低HDL-C对照组比较,PON-1活性无明显差别,但PON-1/apoA-Ⅰ比值显著降低(P<0.05);③LPL-/-鼠与对照组比较TG明显升高(P<0.001),PON-1活性显著下降(P<0.001)。结论:血清PON-1活性降低反映HDL抗氧化功能的异常,这可能与HTG易致动脉粥样硬化的发生密切相关。Objective：To investigate the alteration of the activity of paraoxonase-1（PON-1）,an apoli-poprotein AⅠ（apoAⅠ）-associated arylesterase which located on the high density lipoprotein （HDL）,in hypertriglyceridemia（HTG）.Methods：The fasting plasma samples were obtained from 40patients with HTG patients and 65 control individuals of Wuhan,Hubei province,the plasma lipid profile including triglycerides （TG）,total cholesterol（TC）,HDL cholesterol （HDL-C）,low-density lipoprotein cholesterol（LDL-C）,apoA-Ⅰand apoB100were determined enzymatically,and plasma PON-1activity was measured by monitoring the increase of absorbency at 405nm using paraoxon as the substrate.Lipoprotein lipase gene knockout（LPL-/-）mice as HTG models were also detected.Results：Compared with control subjects,the level of plasma HDL-C and apoA-Ⅰof HTG group were lower（P0.05）,PON-1activity was also lower obvi-ously（P0.001）,and the level of apoB100was higher（P0.01）;plasma PON-1activity was not significantly different between lower HDL-C with normal TG group and HTG with normal HDL-C group,but PON-1/apoA-Ⅰ ratio significantly decreased in HTG group（P0.05）;com-pared with that respectively in wild type mice,significantly higher TG and lower PON-1 activity were detected in LPL-/-mice（P0.001）.Conclusion：The decrease of plasma PON-1activity that reflects abnormality of HDL anti-oxidation might be closely relative to atherogenesis in HTG patients.

关 键 词：高甘油三酯血症 对氧磷酯酶-1 高密度脂蛋白 脂蛋白酯酶基因敲除鼠 

分 类 号：R541.4[医药卫生—心血管疾病]