呼吸道合胞病毒激活RIG-Ⅰ/TLR3信号通路及对SOCS1/3 mRNA表达的影响  

Activating of RIG-Ⅰ/TLR3 Signal Pathway and Its Impact on the Expression of SOCS1/3 mRNA After Respiratory Syncytial Virus Infection

作　　者：杨坤[1] 汪霞[1] 魏聪[1] 黄媛[1] 付学东[1] 赵东赤[1] 

机构地区：[1]武汉大学中南医院儿科,湖北武汉430071

出　　处：《武汉大学学报（医学版）》2010年第5期571-575,共5页Medical Journal of Wuhan University

基　　金：国家自然科学基金资助项目(编号:30973220)

摘　　要：目的:探讨视黄酸诱导基因Ⅰ(RIG-Ⅰ)和Toll样受体3(TLR3)两受体在呼吸道合胞病毒(RSV)感染A549细胞后诱导的炎症和抗病毒反应中的作用。方法:应用Real-timePCR方法检测RSV感染A549细胞后0,2,4,8,24hRIG-Ⅰ,TLR3及细胞因子信号转导抑制因子1/3(SOCS1/3)的mRNA的表达水平;用特异性的si-RNA分别抑制RIG-Ⅰ和TLR3两个受体基因的表达,应用Real-timePCR方法检测RSV感染抑制后的A549细胞不同时间点SOCS1/3的mRNA的表达水平。结果:RSV上调RIG-Ⅰ/TLR3两受体及SOCS1/3mRNA的表达(P<0.05);抑制RIG-Ⅰ及TLR3后,SOCS1/3mRNA的表达与对照组相比无差异性(P>0.05)。结论:RSV感染A549细胞后上调负性调节因子SOCS1/3的表达,其表达不依赖RIG-Ⅰ及TLR3受体途径,可能由病毒复制直接诱导。Objective：To explore the roles of retinoic acid-inducible gene-Ⅰ（RIG-Ⅰ）and Toll-like receptor 3（TLR3）in respiratory syncytial virus（RSV）-induced inflammatory and antiviral responses of A549cells.Methods：A549 cells were infected with RSVin vitro,and the cells were harvested at 0,2,4,8,24 hpost-infection respectively,then real-time PCR was used to detect the expression of RIG-Ⅰ/TLR3 and SOCS1/3mRNA;specific siRNA was used to inhibit the expres-sion of RIG-Ⅰand TLR3receptors before A549 cells were infection by RSV,total cell RNA was collected for real-time PCR to detect the expression of SOCS1/3 mRNA at different time.Results：After infection with RSV,the expression of RIG-Ⅰ/TLR3 and SOCS1/3 mRNA was up-regulated（P0.05）.After siRNA inhibition of RIG-Ⅰand TLR3respectively,the expression of SOCS1/3 mRNA had no changes as compared with that of the control（both P0.05）.Conclusion：RSV up-regulated the expression of SOCS1/3 mRNA through a RIG-Ⅰ-independent and TLR3-independent pathway,and it may be divectly induced by viral replication.

关 键 词：呼吸道合胞病毒 A549细胞 视黄酸诱导基因I TOLL样受体3 细胞因子信号转导抑制因子 

分 类 号：R725.6[医药卫生—儿科]