当归补血汤对大鼠糖尿病足iNOS mRNA表达的影响  

Protective Effect of Danggui Buxue Decoction in Rats with Diabetic Foot

作　　者：杨利剑[1] 张莹雯[2] 

机构地区：[1]武汉理工大学医院,湖北武汉430070 [2]武汉大学中南医院中西医结合科,湖北武汉430071

出　　处：《武汉大学学报（医学版）》2010年第5期592-595,共4页Medical Journal of Wuhan University

摘　　要：目的:探讨当归补血汤对糖尿病大鼠足部病变的保护作用及其机制。方法:SD雄性大鼠腹腔注射链脲佐菌素(STZ)并结合室温控制以建立糖尿病足模型,给予当归补血汤或西洛他唑治疗。实验第3周末时,观察各组肢端坏疽并测定血糖情况。腹主动脉取血,测定血甘油三酯(TG)、胆固醇(TC)、高密度脂蛋白(HDL-C)、C反应蛋白(CRP)、C肽(CP)、糖化血红蛋白(GHb)。应用反转录多聚酶链反应技术(RT-PCR),观察各组大鼠小腿腓肠肌iNOSmRNA的表达情况。结果:STZ诱导的糖尿病大鼠存在明显的血糖、血脂及CRP、CP、GHb异常(P<0.01或<0.05),且出现不同程度的肢端坏疽。当归补血汤可以降低上述指标,减轻肢端坏疽(P<0.01或<0.05),而西洛他唑只改善了CRP和CP(P<0.01)。经给药处理3周后,当归补血汤组及西洛他唑组大鼠足部iNOSmRNA均有不同程度的下调(P<0.01)。结论:当归补血汤可改善血糖、血脂代谢紊乱,降低异常升高的GHb、CP、CRP水平,减轻肢端坏疽的症状,并能纠正升高的iNOSmRNA的表达,推测其能延缓高糖状态下的足部病变,对糖尿病足有一定的保护作用。Objective：To explore the protective mechanism of Danggui Buxue Decoction（DBD）,a traditional Chinese drug which consists of radix astragali and angelica sinensis,in rats with diabetic foot.Methods：The rat model with diabetic foot was made by intraperitoneal injection of 55 mg/kg streptozotocin（STZ）and temperature control.Rats were treated with DBD or cilostazol.After 3weeks of treatment,blood glucose,lipid,glycohemoglobin（GHb）,C-peptide（CP）and C-reactive protein（CRP）were measured,and gangrene of feet was also observed.RT-PCR technique was used to observe the expression of iNOS mRNA.Results：The diabetic rats induced by STZ had abnormal blood levels of glucose,lipid,GHb,CP,and CRP（P0.01or 0.05）,and different degrees of gangrene.DBD alleviate all these changes（P0.01or 0.05）,while cilostazol only lowered blood CP and CRP levels（P0.01）.The level of the expression of iNOS mRNA was obviously higher in diabetic rats（P0.01）,but DBD and cilostazol could decrease it （both P0.01）.Conclusion：DBD may alleviate the disorder of blood glucose and lipid,lowerthe levels of GHb,CP and CRP,inhibit the expression of iNOS mRNA.These effects may slow down the progress of diabetic foot.

关 键 词：当归补血汤 糖尿病足 一氧化氮合酶 大鼠 

分 类 号：R587.2[医药卫生—内分泌]