骨髓基质干细胞复合藻酸钙凝胶修复全层半月板无血运区缺损  

Repair full-thickness meniscal defects with injectable tissue engineering technique

作　　者：张海宁[1] 冷萍[1] 王英振[1] 吕成昱[1] 王湘达[1] 王昌耀[1] 

机构地区：[1]青岛大学医学院附属医院关节外科,266003

出　　处：《中华外科杂志》2010年第17期1309-1312,共4页Chinese Journal of Surgery

基　　金：国家自然科学基金资助项目（30801167）

摘　　要：目的 观察骨髓基质干细胞复合藻酸钙凝胶注射式修复全层半月板无血运区缺损的效果.方法 2008年6月至2009年2月制造成年山羊半月板前角无血运区全层缺损模型.以自体骨髓基质干细胞复合可注射藻酸钙凝胶修复半月板缺损（Ⅰ组）,同时设立单纯载体组（Ⅱ组）和空白对照组（Ⅲ组）.术后4、8、16周处死动物,行大体观察,组织学观察、电镜观察和MRI检查并比较修复效果.结果 Ⅰ组缺损完全被修复组织填充,结合紧密,与正常半月板组织相似,在4～16周效果逐渐改善,大体观察优于其他各组.光镜见细胞随凝胶纤维排列分布,载体纤维间隙大多为细胞分泌基质所充填,细胞排列密集,基质分布均匀.透射电镜见Ⅰ组细胞呈软骨细胞样形态,细胞突起较多,细胞器丰富,细胞为不同走向排列的纤维包绕.MRI检查发现Ⅰ组修复效果较好.结论 骨髓基质干细胞复合藻酸钙凝胶注射可有效地修复全层半月板无血运区缺损.Objective To investigate the effectiveness of injectable tissue engineering to repair fullthickness meniscal defects. Methods From June 2008 to February 2009 full-thickness of meniscal defects were created in the anterior corner of goats, which with no blood supply, in a diameter of 2 mm. Then bone marrostem cells（BMSCs） was mixed with injectable calcium alginate gel to fill the defects. Other groups include the calcium alginate gel and empty group were served as control groups. At different time points, the animals were sacrificed and macroscopy, microscopy determination, electroscopy and MRI detection were performed to assess the outcomes of repairment. Results The meniscal defects had been filled thoroughly in 16 weeks after operation with white,tough and elastic repair tissue similar to normal meniscal fibrocartilage in the tissue engineering groups. The repair tissue was mainly fibrochondrocytes in line with the calcium alginate fiber. Thick matrix secreted by the cells crammed the space between fibers. The view under electroscopy demonstrated that the microstructure of the repair tissue was normal and cells were in a fibrocartilage phenotype. Conclusion The full-thickness meniscal defects in regions without blood supply can be reconstructed effectively with injectable tissue engineering.

关 键 词：组织工程 半月板 胫骨 缺损 

分 类 号：R318.17[医药卫生—生物医学工程]