大黄酸对SLT-Ⅱe诱导大鼠肠黏膜微血管内皮细胞分泌细胞因子的影响  

Effects of rhein on the secretion of cytokines in rat intestinal microvascular endothelial cells induced by SLT-Ⅱe

作　　者：周宏超[1,2] 刘晓强[1] 刘钟杰[3] 穆祥[2] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100 [2]北京农学院兽医学(中医药)北京市重点实验室,北京102206 [3]中国农业大学动物医学院,北京100094

出　　处：《中国兽医科学》2010年第9期964-969,共6页Chinese Veterinary Science

基　　金：国家自然科学基金项目(30671543);北京市自然科学基金项目(6021001);北京市属市管高等学校人才强教计划项目(BAHED)

摘　　要：通过检测大黄酸对SLT-Ⅱe诱导的大鼠肠黏膜微血管内皮细胞分泌一氧化氮(NO)、内皮素-1(ET-1)、前列环素(PGI2)、血栓素A2(TXA2)、P-选凝素及可溶性细胞间黏附分子-1(sICAM-1)的浓度变化,以探索中药复方主要成分大黄酸对仔猪水肿病的疗效机制。将培养的肠黏膜微血管内皮细胞分为对照组、SLT-Ⅱe组、不同浓度大黄酸处理组等5个组,采用ELISA法测定了培养3、6、9和12h时细胞培养上清液中NO、ET-1、PGI2、TXA2、P-选凝素及sICAM-1浓度的变化。结果显示,5μg/mL大黄酸可显著下调SLT-Ⅱe诱导的大鼠肠黏膜微血管内皮细胞NO、P-选凝素及sICAM-1的分泌,12h内使NO、P-选凝素及sICAM-1的分泌浓度接近正常水平,对ET-1的分泌也有一定的抑制作用;1、5、10μg/mL大黄酸不能下调SLT-Ⅱe诱导的大鼠肠黏膜微血管内皮细胞PGI2和TXA2的分泌。表明,大黄酸通过抑制SLT-Ⅱe诱导肠黏膜微血管内皮细胞NO、ET-1、P-选凝素及sICAM-1的过量分泌,缓解肠道微循环障碍,减轻炎症反应,达到治疗水肿病的目的。The concentration of NO,ET-1,PGI2,TXA2,P-selectin and sICAM-1 in intestinal m icrovascular endothelial cells（IMECs）of rats induced by SLT-Ⅱe were determine d in order to study the functional mechanism of rhein on edema disease of pigs.The cultured IMECs in vitro were divided into control group,SLT-Ⅱe group,test 1 group,test 2 group and test 3 group.The concentration of NO,ET-1,PGI2,TXA2,P-selectin and sICAM-1 in cell superna tant which were cultivated for 3,6,9 and 12 h were detected using ELISA respect ively.The results showed that 5 μg/mL rhein reduced the secretion of NO,ET-1,P-sel ectin and sICAM-1 remarkably.The secretion of PGI2 and TXA2 was not reduced by 1 μg/mL,5 μg/mL and 10 μg/mL concentrations of rhein.In conclusion,rhein could reduce the overfull secretion of NO,ET-1,P-selecti n and sICAM-1 in IMECs of rat,relive intestinal microcirculation obstruction,alleviate the inflammation response and treat edema disease of swine.

关 键 词：志贺样毒素Ⅱ型变异体 大鼠 肠黏膜微血管内皮细胞 一氧化氮 内皮素-1 前列环素 血栓素A2 P-选凝素 可溶性细胞间黏附分子-1 大黄酸 

分 类 号：S852.21[农业科学—基础兽医学]