调节性T细胞和Th17细胞比值的变化与特发性血小板减少性紫癜发病关系的研究  

Expression of regulatory T cells and Th17 cells in idiopathic thrombocytopenic purpura and its significance

作　　者：郭宁红[1] 石庆之[1] 华建媛[1] 李振江[1] 李剑[1] 贺文凤[1] 吴琼[1] 

机构地区：[1]南昌大学第二附属医院血液内科,330006

出　　处：《中华血液学杂志》2010年第9期610-612,共3页Chinese Journal of Hematology

摘　　要：目的 探讨调节性T细胞和Th17细胞比值在特发性血小板减少性紫癜（ITP）发病机制中的作用.方法 应用流式细胞术检测29例ITP患者和28名正常人外周血中CD4^＋CD25^＋T细胞、CD4^＋CD25^high T细胞、CD4^＋IL-17A＋T细胞的数量,ELISA法测定血浆中IL-10、IL-17水平;RT-PCR法检测外周血Foxp3和RORc mRNA的表达水平.结果 与正常对照组比较,ITP患者外周血CD4^＋CD25^＋T细胞/CD4^＋T细胞比值升高（P＜0.05）,CD4^＋CD25^high T细胞/CD4＋T细胞比值下降（P＜0.05）,CD4＋IL-17A＋T细胞/CD4^＋T细胞比值差异无统计学意义（P＞0.05）,CD4^＋CD25^highT细胞/CD4^＋IL-17＋T细胞比值从11.42±5.09降低为1.08±0.84（P＜0.05）.ITP患者血浆IL-10水平明显低于正常对照组（P＜0.05）,IL-17水平与对照组比较无显著差异（P＞0.05）.ITP患者较正常对照组外周血中Foxp3 mRNA水平明显降低（P＜0.05）,而RORc mRNA表达则较对照组明显升高（P＜0.05）.结论 ITP患者存在调节性T细胞/Th17细胞比例平衡失调,其可能在ITP免疫发病机制中起一定作用.Objective To investigate the expression of regulatory T cells and Th17 cells in idiopathic thrombocytopenic purpura （ITP） and its significance. Methods The quantity of CD4^ ＋ CD25^ ＋ T cells,CD4^＋ CD25high T cells and CD4^＋ IL-17＋ T cells in peripheral blood were measured with flow cytometry （FCM）, the plasma level of IL-10 and IL-17 with ELISA and the expression of Foxp3 mRNA and RORc mRNA with RT-PCR in 29 newly diagnosed ITP patients and 28 healthy controls. Results The ratio of CD4 ^＋ CD25^ ＋ T cells/CD4 ^＋ T cells in the peripheral blood of ITP patients was significantly higher than that of the normal controls （P 〈0.05 ）. And the ratio of CD4^ ＋ CD25highT/CD4^ ＋ T cells in the patients was remarkably lower （P〈0.05）. There was no difference in the percentage of CD4^＋ IL-17＋T/CD4^＋ T cells between ITP patients and controls（P 〉0.05 ）. The ratio of Treg/Th17 was lower in ITP patients （ P 〈 0. 05 ）. The plasma level of IL-10 in ITP patients was significantly decreased （P〈0.05）, whereas no statistical difference was observed in the level of IL-17 （P〉0.05）. The Foxp3 mRNA expression levels were largely reduced （P 〈 0.05）, but the RORc mRNA expression was increased compared with that in controls （P〈0.05）.Conclusion The imbalance of Treg/Th17 plays an important role in the pathogenesis of ITP.

关 键 词：T淋巴细胞 调节 辅助诱导 紫癜 血小板减少性 特发性 

分 类 号：R554.6[医药卫生—血液循环系统疾病]