西红花酸对3T3-L1前脂细胞增殖和分化的影响  

Effects of crocetin on the proliferation and differentiation of 3T3-L1 preadipocyte

作　　者：张晓明[1] 钱之玉[1] 季晖[1] 杨丽娜[1] 杨如会[1] 宋磊[1] 

机构地区：[1]中国药科大学药理教研室

出　　处：《中国临床药理学与治疗学》2010年第7期753-757,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的:观察西红花酸对3T3-L1前脂细胞增殖分化的影响,并探讨其影响3T3-L1细胞分化的可能作用机制。方法:培养前脂肪细胞3T3-L1,四甲基偶氮唑盐(MTT)法检测西红花酸对其生长活性和增殖的影响;油红O染色和染色比色法分析脂肪细胞的分化程度;逆转录多聚酶联反应(RT-PCR)检测腺苷酸激活蛋白激酶(AMP-activated protein kinase,AMPK)mRNA的表达;Western blot法检测p-AMPK蛋白表达水平。结果:西红花酸高、中剂量(10-5、10-6mol/L)组均可以显著抑制前脂细胞的增殖和分化(P<0.01),而低剂量组(10-7mol/L)也可以抑制其增殖、分化(P<0.05);各剂量的西红花酸都能够增加AMPK的mRNA的表达、提高p-AMPK蛋白表达水平(P<0.05)。结论:西红花酸具有抑制前脂细胞增殖分化的作用,可能和增强AMPK的表达有关。AIM：To observe the effect of crocetin on proliferation and differentiation of 3T3-L1 preadipocyte, and to investigate the possible mechanism involved. METHODS： The 3T3-L1 cells were pretreated with crocetin, while, the MTT method was used to detect the proliferation of the cells, oil red O staining method and spectrophotography were applied to analyze the degree of differentiation. The expression of AMP-activated protein kinase（AMPK）mRNA, which was called as the energy regulator of cell, was detected by reverse transcription PCR （RT-PCR）, and the protein level of p-AMPK was determined by western blot. RESULTS： High and middle dose crocetin（10-5, 10-6 mol/L） could significantly inhibit the proliferation and differentiation of 3T3-L1 cell（P0.01）; also, the low dose croctin（10-7 mol/L） could inhibit its proliferation and differentiation（P0.05）; croctin could incease the expression of AMPK mRNA and the protein level of p-AMPK（P0.05）. CONCLUSION： Crocetin inhibit the proliferation and differentiation of 3T3-L1 cell possibly by influence AMPK.

关 键 词：西红花酸 3T3-L1细胞 增殖分化 腺苷酸激活蛋白激酶 

分 类 号：R965.2[医药卫生—药理学]