快速灵敏的LC-MS/MS法测定人血浆中缬沙坦浓度  

A rapid and sensitive LC-MS/MS method for determination of valsartan in human plasma

作　　者：李玲[1,2] 谭志荣[1] 陈尧[1] 周淦[1] 郭栋[1] 严谨[3] 阳国平[3] 欧阳冬生[1] 

机构地区：[1]中南大学临床药理研究所 [2]中南大学药学院 [3]中南大学湘雅三医院

出　　处：《中国临床药理学与治疗学》2010年第7期809-813,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的:建立一种快速、灵敏的高效液相色谱-串联质谱法(LC-MS/MS)测定人血浆缬沙坦浓度。方法:200μL血浆样品经乙腈一步沉淀蛋白后,在Inertsil ODS-色谱柱(2.1mm×150mm,5μm)上分离,流动相由乙腈和1‰甲酸水溶液(70∶30)组成。采用电喷雾离子源(ESI源)正离子多反应监测(MRM)扫描分析,缬沙坦和厄贝沙坦的离子选择通道分别为:m/z436.3→235.2和429.4→207.2。结果:缬沙坦的线性范围为24.2～3100.0μg/L,日内和日间相对标准差均小于15%。结论:本法操作快速、灵敏,适用于缬沙坦的临床药动学研究。AIM：To establish a rapid and sensitive LC-MS/MS method for determination of valsartan in human plasma. METHODS： The prepared analytes were separated on an ODS column using mobile phase of acetonitrile and 1‰ methanoic acid （70∶30）. Valsartan and irbesartan were detected by multiple reaction monitoring （MRM） mode after ionized by ESI source. The transitions of m/z436.3→235.2 and m/z 429.4→207.2 were used to quantify valsartan and irbesartan, respectively. RESULTS：The method was linear in the range of 24.2-3100.0 μg/L for valsartan. The intra-day mean precision and inter-day mean accuracy precision was not more than 15%. CONCLUSION： The method is rapid and sensitive, and it＇s adaptive for clinic pharmacokinetic evaluation of valsartan.

关 键 词：缬沙坦 液质联用法 生物等效性 

分 类 号：R969.1[医药卫生—药理学]