姜黄素对APP/PS1转基因鼠骨微结构及骨密度的影响  

Effects of curcumin on bone microarchitecture and bone mineral density by APP /PS1 transgenic mice

作　　者：杨茂伟[1] 王铜浩[1] 颜培培[2] 初立伟[1] 高志达[1] 

机构地区：[1]中国医科大学附属第一医院骨科,沈阳110001 [2]中国医科大学附属第一医院内分泌科

出　　处：《中国骨质疏松杂志》2010年第9期647-651,共5页Chinese Journal of Osteoporosis

基　　金：辽宁省教育厅科研基金(L2010590)

摘　　要：目的应用Micro-CT定量研究用姜黄素治疗前后APP/PS1转基因鼠胫骨近端的骨微结构及骨密度的变化。方法 3月龄雌性小鼠(n=6/组)分为以下3组:野生型小鼠(对照组),APP/PS1转基因鼠(模型组)和APP/PS1转基因鼠+姜黄素(用药组)。对照组和模型组自由摄食和饮水;用药组,姜黄素按600mg·kg-1加入正常小鼠饲料,3组小鼠喂养至12月龄时处死,取其胫骨,应用Micro-CT进行扫描分析。结果 APP/PS1转基因组小鼠胫骨近端骨微结构、骨密度参数明显小于野生型小鼠(P<0.05);APP/PS1转基因小鼠用药后胫骨近端骨微结构、骨密度参数明显大于用药前(P<0.05),接近于野生型小鼠(P>0.05)。结论姜黄素有助于提高APP/PS1转基因组小鼠的骨小梁数目、骨小梁厚度,骨密度,对APP/PS1转基因组小鼠骨微结构的改善有一定的治疗作用。Objective To quantitatively investigate changes in bone mieroarchitecture and hone mineral density of the proximal tibia by APP/PS1 transgenic mice exposed to Curcumin using micro-computerized tomography. Methods 3-month-old female mice （6/group） were divided into following groups： wild-type mice （control group）, APP/PS1 transgenic mice （model group）, and APP/PS1 transgenic mice with Curcumin treatment （Curcumin group）. Mice in control group and model group were feed ad libitum. Mice in Curcumin group were administrated with Curcuminorally （600 mg/kg）. Mice in all 3 groups were saerifced at 12 months of age and the tibia were harvested to analyze the bone microarchitecturc and bone mineral density using micro-CT. Results Bone microarchitecture and bone mineral density of APP/PS1 transgenic mice were significantly lower than those of wild-type mice （ P 〈 0.05 ）. After treatment of Curcumin,the above parameters were significantly larger than those before treatment （ P 〈 0.05 ） , and were close to the levels of wild-type mice （ P 〉 0.05 ）. Conclusion Curcumin increased trabecular number, trabecular thickness, bone mineral density and produced beneficial effects on bone microarchitecture in APP/PS1 transgenic mice.

关 键 词：姜黄素 APP/PS1转基因鼠 micro—CT 骨微结构 骨密度 

分 类 号：R282.71[医药卫生—中药学]