非洲爪蟾短型肽聚糖识别蛋白基因的克隆与鉴定  

CLONING AND IDENTIFICATION OF A SHORT TYPE PEPTIDOGLYCAN RECOGNITION PROTEIN IN XENOPUS TROPICALIS

作　　者：齐志涛[1,2,3] 高谦[1] 黄贝[1,3] 昌鸣先[1] 聂品[1] 

机构地区：[1]中国科学院水生生物研究所,淡水生态与生物技术国家重点实验室,武汉430072 [2]盐城工学院海洋技术系,江苏省沿海池塘养殖生态重点实验室,盐城224051 [3]中国科学院研究生院,北京100049

出　　处：《水生生物学报》2010年第5期922-926,共5页Acta Hydrobiologica Sinica

基　　金：国家自然科学基金资助项目(30830083);中国科学院创新基金(KSCX2-YW-N-021)资助

摘　　要：采用生物信息学方法首次对非洲爪蟾短型肽聚糖识别蛋白(xePGRP-S)基因进行了克隆,并对其在胚胎发育和成年爪蟾各组织中的表达状况进行了分析。xePGRP-ScDNA全长720bp,开放阅读框为549bp,编码182个氨基酸。序列比对显示xePGRP-S与其他物种PGRP-S的序列相似性在42.4%—50.5%之间。RT-PCR显示在非洲爪蟾胚胎发育至3d时可以明显检测到xePGRP-S的表达,之后呈持续性表达,且在所检测的心、肝、脾、肺、肾、肠和胃这7种组织器官中呈组成型表达。A short type peptidoglycan recognition protein （PGRP-S） was first cloned from the amphibian model animal Xenopus tropicalis by using the bioinformatics method, and the expression pattern of xePGRP-S in early developing stage and normal xenopus was also analyzed. The xePGRP-S cDNA was 720 bp in length, containing a 549 bp open reading frame encoding 182 a.a. Multiple sequence alignment showed that the xePGRP-S shared 42.4%—50.5% sequence identities with other PGRP-S. RT-PCR showed that xePGRP-S mRNA was not detected in the early development stages （3—48h） until the third day. In the normal adult xenopus, xePGRP-S mRNA was constitutively expressed in all the detected tissues including heart, liver, spleen, lung, kidney, intestine and stomach.

关 键 词：非洲爪蟾 肽聚糖识别蛋白 克隆 同源性 组织表达 

分 类 号：S917.4[农业科学—水产科学]