去势8周大鼠松质骨定量CT与化学位移成像  

The study of postoperative rats by quantitative computed tomography and chemical shift imaging

作　　者：李瀛[1] 赵江民[1] 张蕾[1] 刘佳[1] 

机构地区：[1]同济大学附属东方医院医学影像科,上海200120

出　　处：《复旦学报（医学版）》2010年第5期570-574,共5页Fudan University Journal of Medical Sciences

摘　　要：目的检测SD大鼠松质骨定量CT(quantitative computed tomography,QCT)、化学位移成像(chemical shift imaging,CSI)的定量指标在去势8周后的变化,探讨这两项指标对去势后松质骨改变的监测意义。方法 12周龄SD大鼠38只,雌雄各半,分为4组,雌性去势组(10只),雌性对照组(9只),雄性去势组(10只),雄性对照组(9只)。术后8周行QCT松质骨容积骨密度(volumetric bone mineral density,VBMD)检测和CSI计算化学位移率(chemical shift rate,CSR),影像学检查后进行髂骨区域松质骨取样,病理光镜下分析细胞构成比,计算脂肪含量(fat fraction,%FF)。结果去势8周后,雌、雄大鼠去势组与对照组之间,松质骨QCT差异有统计学意义(雌性大鼠:P=0.003<0.01);(雄性大鼠:P=0.011<0.05);雌性大鼠去势组与对照组之间,松质骨CSR差异有统计学意义(P=0.001<0.01)。大鼠松质骨QCT、CSR均与松质骨骨髓脂肪含量呈负相关关系,CSR与QCT-VBMD呈正相关关系。结论去势8周,大鼠松质骨脂肪含量增加,骨密度减少,CSR随着骨密度的降低而减少。Objective To evaluate quantitative computed tomography（QCT） and chemical shift imaging（CSI） changes in the postoperative ovariectomized（OVM）/orchidectomy（ORM） SD rats,correlating with the fat content of bone marrow. Methods Nineteen female SD rats and 19 male SD rats at 12 weeks old were randomized to 4 groups： 10 ovariectomized（OVM） female rats,10 orchidectomy（ORM） male rats,and 18 sham-operated（SHAM） rats.Eight weeks after operation,rats were examined by QCT and CSI.The bone of sacrificed rats were processed non-calcified sections and stained with HE stain,then were analyzed with grid in order to get marrow＇s histological characteristics. Results At the time of post-operation 8 weeks,the differences of QCT between the OVM and sham group were statistically significant（P0.01 in female rats,P0.05 in male rats）.The CSR between the OVM and sham group was significantly different only in female rats（P0.01）.A negative correlation was found between QCT and %FF.A negative correlation was found between CSR and %FF.A positive correlation was found between CSR and QCT. Conclusions QCT could show the difference between the SHAM and the OVM/ORM rats 8 weeks later after the operation.In postoperative rats,the QCT and CSR value reduced with the increasing of bone %FF.The CSR value reduced as bone mineral content reduced.

关 键 词：去势 定量CT 磁共振成像 化学位移成像 

分 类 号：R445.2[医药卫生—影像医学与核医学]