氨基葡萄糖对小鼠腹腔巨噬细胞免疫功能的影响  

Effect of Glucosamine on Immune Function of Murine Peritoneal Macrophages

作　　者：蔡文娣[1] 初金鑫[1] 付辰炜[2] 刘万顺[2] 韩宝芹[2] 

机构地区：[1]潍坊医学院基础医学教学部,山东潍坊261053 [2]中国海洋大学海洋生命学院,山东青岛266003

出　　处：《中国生物制品学杂志》2010年第9期946-948,952,共4页Chinese Journal of Biologicals

基　　金：国家高技术研究发展计划(863计划)(2003AA625050)

摘　　要：目的探讨氨基葡萄糖对小鼠腹腔巨噬细胞(PMФ)免疫功能的影响。方法用壳聚糖制备氨基葡萄糖纯品,通过体外实验测定其对小鼠PMФ增殖、吞噬中性红、产生NO和IL-1能力的影响。结果氨基葡萄糖能够显著增强PMФ对中性红的吞噬能力,提高NO和IL-1的生成量,而对PMФ的增殖能力无明显影响。结论氨基葡萄糖能够活化PMФ,增强小鼠的免疫功能。Objective To investigate the effect of glucosamine（GlcNH2） on immune function of murine peritoneal macrophages（PMФ）.Methods Pure GlcNH2 was prepared with chitosan,of which the effects on proliferation as well as abilities in phagocytosis of neutral red and producing NO and IL-1of PMФ were determined by in vitro tests.Results GlcNH2 enhanced the phagocytosis of neutral and increased red the production of NO and IL-1 by PMФ significantly,while showed no significant effect on the proliferation of PMФ.Conclusion GlcNH2 activated PMФ and enhanced the immune function of mice.

关 键 词：氨基葡萄糖 巨噬细胞 细胞增殖 中性红 一氧化氮 白细胞介素-1 

分 类 号：R965[医药卫生—药理学]