一种新型脂肽的分离纯化及其生物学活性  

Isolation,Purification and Bioactivity of A Novel Lipopeptide

作　　者：杨燕[1] 张洪丹[1] 王小溪[1] 蒋子威[1] 黄静[1] 赖玉平[1] 吴自荣[1] 

机构地区：[1]华东师范大学生命科学学院,上海200063

出　　处：《中国生物制品学杂志》2010年第9期982-985,共4页Chinese Journal of Biologicals

摘　　要：目的从表皮葡萄球菌发酵液中分离纯化脂肽粗提物,并分析其生物学活性。方法通过酸沉淀分离和有机溶剂抽提的方法,从表皮葡萄球菌1457发酵液中得到脂肽粗提物。利用排油圈试验和薄层色谱(TLC)分析脂肽粗提物的性质,HPLC分析其纯度。小鼠皮下注射脂肽粗提物后,再经皮肤感染金黄葡萄球菌,检测其对小鼠体内白细胞的影响及抑菌作用;实时定量PCR检测其对小鼠体内β防御素14(mBD14)表达的影响。结果所得脂肽粗提物F2的排油活性大于F1;TLC分析显示,F2中存在性质相近的脂肽类同系物,纯度>60%;脂肽粗提物能减缓小鼠因感染金黄葡萄球菌引起的急性炎症,降低由感染引起的白细胞增多,并通过上调mBD14的表达抑制细菌在皮肤内的增殖。结论已从表皮葡萄球菌发酵液中成功获得了具有生物学活性的新型脂肽粗提物。Objective To isolate and purify crude lipopeptide from the fermentation liquid of Staphylococcus epidermidis and analyze its bioactivity.Methods The crude extracts of lipopeptide were obtained from the fermentation liquid of Staphylococcus epidermidis by acid precipitation and organic solvent extraction,and analyzed for properties by oil-drain test,thin layer chromatography（TLC）and HPLC.Mice were percutaneously infected with Staphylococcus aureus then injected s.c.with the crude extracts to observe the effect on leukocytes in vivo as well as anti-bacterial activity.The effect of the lipopeptide on expression of β-defensin14（mBD14）in mice were determined by real-time quantitative PCR.Results The activity of crude extract F2 of lipopeptide in oildrain was higher than that of extract F1.TLC showed homologue of lipopeptide,with a purity of more than 60%,in extract F2.The extracts relieved the acute inflammation and leucocytosis of mice caused by infection with Staphylococcus aureus,and inhibited the proliferation of bacteria in skin by up-regulating the expression of mBD14.Conclusion The crude extract of a novel lipopeptide with bioactivity was successfully obtained from the fermentation liquid of Staphylococcus epidermidis.

关 键 词：葡萄球菌 表皮 脂肽 葡萄球菌 金黄色 Β防御素 

分 类 号：R392.33[医药卫生—免疫学]