紫外分光光度法测定双山颗粒中总黄酮的含量  

Determination of the Total Flavones in Shuangshan Granules

作　　者：陈勇[1] 韦韬[1] 魏后超[1] 卢森华[1] 曾海生[1] 巫繁菁[1] 韦玉燕[1] 

机构地区：[1]广西中医学院,广西南宁530001

出　　处：《广西科学院学报》2010年第3期277-279,共3页Journal of Guangxi Academy of Sciences

基　　金：广西科技厅科技项目(合同号:桂科产0815010-7G)资助

摘　　要：采用紫外分光光度法,以芦丁为对照品,对其进行络合显色,在500nm波长处测定吸光度,测定双山颗粒剂中总黄酮的含量。结果显示,芦丁对照品的线性范围为8.04～48.24μg/ml(r=0.9996),总黄酮的平均回收率为98.30%(RSD=18.6%,n=6)。紫外分光光度法简便、准确,可以作为双山颗粒剂的质量控制方法。The method for determination of the total flavones in Shangshan granules was established.Rutin was selected as reference material,and the samples were determined at the wavelengh of 500nm.Rutin showed a good linear relationship with absorbance when its concentration was in the range of 8.04～48.24μg/ml（r=0.9996）.The average recovery of rutin was 98.30%（RSD=18.6%,n=6）.This method is simple,accurate,which can be used for quality control of Shuangshan Granules.

关 键 词：分光光度法 总黄酮 含量 双山颗粒 

分 类 号：R284.1[医药卫生—中药学]