3种黄酮类小分子与牛血清白蛋白间相互作用及其构效关系的研究  

Study of Interaction and Structure-Activity Relationships between Three Flavonoids and BSA

作　　者：陶慧林[1] 寿红娟[1] 朱仕毅[1] 

机构地区：[1]桂林理工大学化学与生物工程学院,广西桂林541004

出　　处：《广西科学院学报》2010年第3期338-342,共5页Journal of Guangxi Academy of Sciences

基　　金：广西教育厅科研项目(No.200708LX339)资助

摘　　要：解析3种黄酮类小分子桑色素(MOR)、槲皮素(QUE)、芦丁(RUT)与牛血清白蛋白(BSA)相互作用后的紫外-可见光谱和荧光猝灭光谱,研究在同一实验条件下黄酮类小分子与BSA的相互作用及其构效关系。结果显示,黄酮母核B环上的羟基处于相邻位置时,比处于其他位置时表现出的BSA猝灭作用和结合作用更强,而有较大取代基团存在时,由于位阻较大,黄酮类小分子与BSA间的猝灭作用及结合作用则明显变弱。The structure-activity relationship and interaction between three flavonoids including Morin（MOR）,Quercetin（QUE） and Rutin（RUT） and BSA were observed by UV-visible spectra and fluorescence quenching spectra.The results showed that the quenching and binding effect between flavonoids and BSA were strong when the.hydroxyl group was in the adjacent position of B benzene ring of nucleus.However,the quenching and binding effect between flavonoids and BSA decreased when a large group existed,which is due to the steric hindrances.

关 键 词：黄酮 牛血清蛋白 相互作用 构效关系 

分 类 号：R965.1[医药卫生—药理学]