运用RAPD技术分析3种罗非鱼的遗传多样性  

Population Genetic Analysis on 3 Species of Tilapias by Random Amplified Polymorphic DNA

作　　者：刘丽[1] 李晓娜[1] 汤恩普 

机构地区：[1]广东海洋大学水产学院南海水产经济动物增养殖广东省普通高校重点实验室,广东湛江524025

出　　处：《广东海洋大学学报》2010年第4期36-38,共3页Journal of Guangdong Ocean University

基　　金：湛江市科技招标项目"水产经济动物新品种(星洲银罗非鱼)的引进与繁殖养成示范"

摘　　要：应用RAPD技术,从7个系列140种随机引物中筛选出15个,对尼罗罗非鱼(Oreochromis niloticus)、奥利亚罗非鱼(O.aureus)和星洲银罗非鱼(O.sp.)进行遗传多样性分析。结果表明,尼罗罗非鱼、奥利亚罗非鱼、星洲银罗非鱼的多态位点比例(P)分别为54.7%、44.4%和56.0%,种内个体间遗传距离分别为0.145 0、0.115 6和0.141 3,种内遗传相似系数分别为0.855 0、0.884 6和0.858 7;种间遗传距离的聚类分析表明,星洲银罗非鱼与尼罗罗非鱼亲缘关系较近,一定程度上验证了星洲银罗非鱼的分类地位。Genetic diversity and Molecular markers of Oreochromis niloticus,O.aureus and O.sp.were studied by Random Amplified Polymorphic DNA（RAPD） technique and 15 random primers were analyzed in this experiment.The result shows that the polymorphic loci ratio（P） were 54.7%,44.4%and 56.0%,the genetic distances of intra-species（D） were 0.145 0,0.115 6 and 0.141 3,and the genetic similarity indexes of intra-species（I） were 0.8550,0.8846 and 0.8587.The relationship between O.sp and O.niloticu is closer in 3 species of Tilapias based on genetic distance.

关 键 词：尼罗罗非鱼 奥利亚罗非鱼 星洲银罗非鱼 遗传多样性 RAPD 

分 类 号：Q953.3[生物学—动物学]