CD4^＋Foxp3^＋调节性T细胞对断烟大鼠肺部炎症及肺气肿的作用  

CD4^＋ Foxp3^ ＋ regulatory T cells in inflammation and emphysema after smoking cessation in rats

作　　者：邱诗林[1] 白晶[1] 钟小宁[1] 黄秋嫔[1] 陈慧[1] 柳广南[1] 

机构地区：[1]广西医科大学第一附属医院呼吸内科,南宁530021

出　　处：《中华结核和呼吸杂志》2010年第9期688-692,共5页Chinese Journal of Tuberculosis and Respiratory Diseases

基　　金：国家自然科学基金（30860106）;广西自然科学基金（2010GXNSFB013060）,志谢 广西壮族自治区人民医院蓝娇医生提供流式细胞仪并帮助测定

摘　　要：目的观察香烟暴露及断烟后大鼠Treg细胞的变化，探讨Treg细胞与断烟后大鼠肺部炎症反应和肺气肿持续存在的关系。方法将50只雄性Wistar大鼠按随机数字表法分为5组：健康对照组、香烟暴露组和断烟组，其中健康对照组和香烟暴露组又分为12周组（对照1组和实验1组）和24周组（对照2组和实验2组）。香烟烟雾暴露法建立大鼠肺气肿模型。HE染色观察大鼠肺气肿的改变，酶联免疫吸附法检测大鼠BALF中白细胞介素（IL-8）和肿瘤坏死因子-α（TNF-α）水平，流式细胞术检测大鼠外周血和肺实质中CD4^±Foxp3调节性T细胞（Treg细胞）比例，实时定量PCR法检测大鼠外周血和肺实质中Foxp3的mRNA表达。多组间比较采用单因素方差分析，组内两两比较采用SNK和Games—Howell检验。结果实验1组和实验2组平均内衬间隔（MLI）为（64．9±5．3）μm和（77．9±11．5）μm，均明显高于对照1组和对照2组的（39．0±3．8）μm和（40．3±2．7）μm，差异均有统计学意义（P〈0．01）；断烟组MLI[（71．5±5．8）μm]介于实验1组和实验2组之间，但肺气肿程度较实验1组明显加重，差异有统计学意义（P〈0．01）。实验1组和实验2组IL-8水平[（68±17）ng／L和（85±16）ng／L]及TNF-α水平[（14．1±1．8）ng／L和（20．1±8．7）ng／L]均明显高于对照1组和对照2组[（44±8）ng／L、（43±9）ng／L及（6．3±2．3）ng,／L、（5．8±1．6）ng／L]，差异均有统计学意义（P〈0．05）。实验1组和实验2组肺实质中CD4^＋Foxp3^＋Treg细胞[（6．6±0．8）％和（5．3±0．9）％]明显少于对照1组和对照2组[（9．0±1．0）％和（9．6±0．9）％]，差异均有统计学意义（P〈0．01）；断烟组肺实质中CD4^＋Foxp3^＋Treg细胞[（7．2±0．6）％]与实验1组比较虽无明显减少，但未能恢复至正常水平。实验1组和实验2组肺实质中Foxp3的mRNA表达量（Objective To evaluate the expression of Treg in a cigarette smoke-induced rat model of emphysema and after smoking cessation in the rats. Methods Fifty male Wistar rats were randomly divided into control group 1 （12 weeks）, control group 2 （24 weeks）, smoke-exposure group 1 （12 weeks）, smoke-exposure group 2 （24 weeks） and smoking cessation group, with 10 rats in each group. Alveolar airspace enlargement was observed by hematoxylin-eosin （HE） staining. IL-8 and TNF-α levels in bronchoalveolar lavage fluid （BALF） were tested by ELISA. The proportion of CD4^＋Foxp3^＋Treg in peripheral blood and lungs of rats was determined by flow cytometry. The mRNA expression of Foxp3 was measured by real-time PCR. Comparisons of the data between different groups were performed using one-way ANOVA, and SNK and Games-Howell test was used for comparison between 2 groups. Results The mean linear intercept （MLI） in smoke-exposure group 1 and group 2 [ （64. 9 ± 5.3 ） μm, （77.9 ± 11.5 ） μm ] was higher than those in the control group 1 and group 2 [ （39. 0 ±3.8） μm, （40. 3±2. 7） μm], all P 〈0. 01. Compared with smoke-exposure group 2, the MLI in smoking cessation group （71.5 ±5.8）μm showed a lower value （ P 〈 0. 01 ）, but still higher than that in smoke-exposure group 1 （ P 〈 0. 01 ）. The IL-8 and TNF-α levels in BALF of smoke-exposure group 1 and group 2 [ （68± 17） ng/L, （85 ± 16） ng/L], [ （ 14. 1 ± 1.8）ng/L, （20. 1 ±8.7） ng/L] were higher than those in control group 1 and group 2 [ （44 ±8） ng/L, （43±9）ng/L], [（6.3 ±2.3） ng/L, （5.8±l.6）ng/L], all P〈0.05. The IL-8 and TNF-α levels were not statistically different between in smoking cessation group （56 ± 6）ng/L, （14. 7 ± 4.7 ）ng/L and smoke-exposure group 1. The percentage of Treg in the lungs of smoke-exposure group 1 and group 2 [ （6. 6 ± 0. 8 ） % , （5.3 ± 0. 9） % ] was significantly decreased as compared to control group 1 and group 2 [ （9. 0 �

关 键 词：T淋巴细胞 调节性 烟 戒烟 肺气肿 

分 类 号：R563.1[医药卫生—呼吸系统] R563.3[医药卫生—内科学]