羊传染性脓疱病毒湖北株的鉴定及分子特征分析  

Identification and Molecular Characteristic Analysis of Orf Virus(ORFV) Hubei Strain

作　　者：张克山[1] 何继军[1] 尚佑军[1] 郭建宏[1] 郑海学[1] 田宏[1] 靳野[1] 刘永杰[1] 王光祥[1] 刘湘涛[1] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部畜禽病毒学重点开放实验室国家口蹄疫参考实验室,兰州730046

出　　处：《畜牧兽医学报》2010年第9期1154-1157,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家现代肉羊产业技术体系(nycytx-39);十一五"国家科技支撑计划课题(2006BAD06A17)

摘　　要：为了确诊疑似羊传染性脓疱病例,分析羊传染性脓疱病毒(Orfvirus,ORFV)的分子特征,作者以湖北某羊场疑似传染性脓疱病料为对象,进行了电镜观察、病理切片染色、特异性目的基因扩增和序列测定及遗传进化关系分析。结果在电镜下观察到ORFV样粒子,切片HE染色可见有炎性细胞浸润,自病料中扩增出特异性目的ORFVB2L基因片段(GenBank序列号GU320351)。结果表明该病例为黑山羊传染性脓疱病毒引起,序列遗传进化分析表明该病毒和2007年分离于中国台湾的毒株(DQ904351)遗传关系最近,和印度2004到2005年分离于绵羊(DQ263306、DQ263305)、山羊(DQ263304、DQ263303)的ORFV同属于一个进化分支。本研究为ORFV分子流行病学研究积累了资料。To identify suspected goats Orf case and analyze the molecular characteristics of Orf Virus（ORFV）,crust materials of lambs with clinical sore mouth symptom from a farm in HuBei Province were used and researched.ORFV in tissue scrapings from the lips was confirmed by electron microscope,HE stain and specific gene（B2L）PCR amplification.Molecular characterization of ORFV strain was performed using amino acid sequence homology comparison and phylogenetic analysis based on B2Lgene.The results showed that this disease was caused by ORFV （named TS/HuB/CHIA/2009）,B2Lgene sequence was submitted to GenBank and obtained accession number GU320351.Phylogenetic tree analysis demonstrated that the GU320351has a closest genetic relationship with DQ904351,which was isolated from Taiwan Province of China in 2007.And GU320351was in the evolutionary branch contain DQ263306,DQ263305,DQ263304 and DQ263303,which was isolated from India in 2004and 2005.Research of this paper accumulated useful material for further studying of molecular epidemiology of ORFV in China.

关 键 词：羊传染性脓疱病毒 鉴定 分子特征分析 

分 类 号：S852.659.1[农业科学—基础兽医学]