西藏日土藏山羊ISSR标记遗传多态性研究  

Analysis of Genetic Diversity in Ritu Tibetan Goats by ISSR

作　　者：王永[1] 王杰[1] 许期树[1] 字向东[1] 欧阳熙[1] 刘鲁蜀[1] 小益西[2] 

机构地区：[1]西南民族大学青藏高原动物遗传资源保护与利用四川省重点实验室,成都610041 [2]西藏自治区农牧科学院,拉萨850000

出　　处：《畜牧兽医学报》2010年第9期1208-1212,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：四川省应用基础项目(2008JY0067-1)

摘　　要：应用ISSR标记分析西藏日土藏山羊的遗传多样性,为其保护和开发利用提供基础资料。从93条ISSR引物中筛选出10条对107只藏山羊进行分析,数据分析利用Excel和SPSS软件完成。结果表明,所筛选出的10条引物特异性较好且多态性较高,共扩增出112条清晰的条带,平均每条引物扩增出11.2条带,其中多态性条带75个,群体多态位点百分率(P)为66.96%,扩增片段大小为219～2534bp,群内个体间遗传相似性指数(s)为0.4600～0.7405,平均0.5647,个体间平均遗传差异(D)为0.4353。根据多态性位点在样本中出现的几率计算出多态性条带的基因频率(f),频率值的范围为0.0455～1.0000。西藏日土藏山羊的遗传多态性较丰富,个体间存在差异,但又具有较好的同质性。Inter simple sequence repeat（ISSR）was used to analyze the genetic diversity of Tibetan goats population in Ritu county.Ten primers were selected from 93ISSR primers and then used to detect the genetic diversity in 107Tibetan goats.The obtained data were analyzed by using Excel 2003and SPSS11.0softwares.Ten out of 93ISSR primers produced 112DNA bands,among them 75bands were polymorphic markers and the size of amplified fragment ranged from 219to 2 534bp.It indicated that the selected primers were specific and could produce enough polymorphic bands.The proportions of polymorphic loci（P）in Ritu Tibetan goats was 66.96%.The genetic similarity index（s）within the detected population ranged from 0.460 0to 0.740 5（with average of 0.564 7）.The averaged individual genetic difference（D）was 0.435 3.The gene frequency （f）ranged from 0.045 5to 1.000 0.These results of ISSR analysis indicated that the Ritu Tibetan goats population presents high level of genetic diversity and genetic variations existed among individuals.

关 键 词：藏山羊 ISSR 遗传多态性 

分 类 号：S813.1[农业科学—畜牧学] S826.83[农业科学—畜牧兽医]