胰岛素样生长因子1对小鼠成骨细胞增殖和碱性磷酸酶活性的影响  

Influence of insulin-like growth factor-1 on mouse osteoblast proliferation and alkaline phosphatase activity

作　　者：郭玲[1] 王敏[2] 郝亮[2] 

机构地区：[1]泸州医学院附属口腔医院修复科,四川省泸州市646000 [2]四川大学华西口腔医学院修复科,四川省成都市610041

出　　处：《中国组织工程研究与临床康复》2010年第33期6095-6098,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：四川省卫生厅基金资助项目(编号:20090201)课题名称:IGF-1与Wnt/β-catenin信号通路在糖尿病颌骨骨质疏松的作用研究~~

摘　　要：背景:胰岛素样生长因子1是骨形成的调节因子,具有促进有丝分裂、促进成骨等作用。由骨细胞所产生的胰岛素样生长因子1在调节成骨细胞和破骨细胞介导的骨骼重建中起着重要的作用。目的:观察胰岛素样生长因子1作用后,小鼠成骨细胞的增殖和碱性磷酸酶的表达。方法:在体外培养的小鼠成骨细胞中分别加入25,50,100及200μg/L的胰岛素样生长因子1,于培养的第1,2,3,4,5天用Cell CountingKit-8比色法检测细胞增殖率;用流式细胞仪检测细胞的增殖周期和凋亡率;于培养的第3,6,9天应用酶联免疫法检测细胞内碱性磷酸酶活性。结果与结论:胰岛素样生长因子1质量浓度在25～200μg/L时,对小鼠成骨细胞的增殖率有明显的促进作用(P<0.05),且呈明显的浓度依赖效应。当胰岛素样生长因子1质量浓度在200μg/L时,能明显提高细胞S期所占的比例。说明胰岛素样生长因子1可加速细胞的增殖活动,以保证参与骨改建的成骨细胞数量而促进骨组织再生。同时,胰岛素样生长因子1在25～200μg/L时,能显著提高细胞内碱性磷酸酶活性(P<0.05),促进成骨细胞分化。BACKGROUND：Insulin-like growth factor-1（IGF-1） is a growth factor to promote mitosis and regulate bone formation.It is of great significance on the dynamic process of osteoclast-driven bone resorption and osteoblast-mediated bone formation.OBJECTIVE：To elucidate the effect of IGF-1 on proliferation of mouse osteoblasts cultured in vitro and alkaline phosphatase activities.METHODS：Mouse osteoblasts cultured in vitro were added into medium containing various concentrations of IGF-1（25,50,100,and 200 μg/L）.Cell proliferation was determined using Cell Counting Kit-8 method;Cell cycle and apoptosis was assayed with flow cytometry.Alkaline phosphatase activity was examined with enzyme-linked immunosorbent assay at 3,6 and 9 days following culture.RESULTS AND CONCLUSION：All IGF-1 concentrations used（25-200 μg/L） were effective to promote the proliferation of mouse osteoblasts（P 0.05）,showing significant concentration-dependent effects.The maximum response was obtained with 200 μg/L,at which of IGF-1 could notably increase the percentage of cells at S phase,which indicated that IGF-1 can accelerate cellular proliferation to ensure the number of osteoblasts that participated bone rebuilding and to promote bone tissue regeneration.Simultaneously,IGF-1 at various concentrations（25-200 μg/L） could significantly enhance alkaline phosphatase activity（P 0.05）,and accelerate osteoblast differentiation.

关 键 词：胰岛素样生长因子1 成骨细胞 增殖 碱性磷酸酶 糖尿病性骨病 

分 类 号：R318[医药卫生—生物医学工程]