羟基喜树碱与人病理性瘢痕成纤维细胞的增殖  

Effect of hydroxycamptothecin on the proliferation of fibroblasts isolated from human pathological scars

作　　者：廖立新[1] 潘登[1] 陈刚泉[1] 李莉[1] 

机构地区：[1]南昌大学第一附属医院烧伤科,江西省南昌市330006

出　　处：《中国组织工程研究与临床康复》2010年第33期6159-6162,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然基金题(30660234)<喜树碱防治病理性瘢痕的实验研究>的一部分~~

摘　　要：背景:课题组前期实验表明病理性瘢痕成纤维细胞中拓扑异构酶Ⅰ存在高表达,实验以此设想作为拓扑异构酶Ⅰ抑制剂的羟基喜树碱能否抑制体外培养的人病理性瘢痕成纤维细胞的增殖?目的:探讨羟基喜树碱对人病理性瘢痕成纤维细胞增殖的影响。方法:体外培养人病理性瘢痕成纤维细胞。选取羟基喜树碱2～1000μg/L共10个药物质量浓度梯度,分别选取给药后24,48和72h时间点,采用MTT法检测羟基喜树碱对成纤维细胞生长的抑制作用。结果与结论:低质量浓度(2～8μg/L)下羟基喜树碱对成纤维细胞的增殖有抑制作用,质量浓度8～125μg/L时出现平台期,质量浓度125～500μg/L时抑制作用进一步增强,说明羟基喜树碱对成纤维细胞的增殖的抑制作用具有明显的剂量依赖性。药物作用质量浓度和成纤维细胞抑制率呈正相关(r=0.87,P<0.05)。随着作用时间延长,细胞抑制率无明显增加,药物作用24,48和72h后,半数抑制率(IC50)分别为233,176及103μg/L。因此实验推论羟基喜树碱能够抑制体外培养的人病理性瘢痕成纤维细胞的增殖。BACKGROUND：Previous experiments have showed that topoisomerase Ⅰ is highly expressed in hypertrophic scar fibroblasts.This experiment hypothesized that weather hydroxycamptothecin（HCPT）,an inhibitor of topoisomerase Ⅰ,can inhibit human hypertrophic scar fibroblasts proliferation cultured in vitro？ OBJECTIVE：To investigate the effect of HCPT on the proliferation of fibroblasts isolated from human pathological scars.METHODS：Pathological scar fibroblasts were cultured in vitro.MTT assay was used to determine the effect of HCPT on the proliferation of fibroblasts under 10 different doses（2-1 000 μg/L） at 24,48 and 72 hours.RESULTS AND CONCLUSION：HCPT with low concentration（2-8 μg/L） exhibited inhibitory effects on fibroblasts proliferation,which reached a platform stage with the next concentration（8-125 μg/L）,and the effect would enhanced at the high concentration（125-500 μg/L）.It revealed that HCPT had inhibitory effects on fibroblasts proliferation in a concentration-dependent manner.The concentration of drug was positively correlated with cell inhibitory rate（r=0.87,P 0.05）.As the time prolonged,the inhibition was not increased.Half of the inhibition rates（IC50） were 233,176 and 103 μg /L at 24,48,and 72 hours.Accordingly,HCPT can inhibit proliferation of human pathological scar fibroblasts in vitro.

关 键 词：羟基喜树碱 成纤维细胞 拓扑异构酶Ⅰ 病理性瘢痕 细胞增殖 皮肤组织构建 

分 类 号：R318[医药卫生—生物医学工程]