视神经组织工程重建中基因治疗工具的作用  

Gene therapy for optic nerve tissue engineering reconstruction

作　　者：郭兵[1] 许家军[1] 

机构地区：[1]解放军第二军医大学人体解剖学教研室,上海市200433

出　　处：《中国组织工程研究与临床康复》2010年第33期6226-6229,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家重点基础研究发展计划(2005CB724302)~~

摘　　要：背景:目前治疗视神经疾病的方法主要有细胞移植治疗,基因治疗,药物治疗,手术干预等。其中,基因治疗研究逐步受到重视,并有望成为某些视神经疾病的可供选择的治疗方法之一。目的:从各种基因载体应用、神经营养因子、组织工程修复以及临床应用等方面,全面了解视神经损伤后基因治疗修复的方法。方法:以"视神经修复(optic nerve regeneration),基因治疗(gene therapy),组织工程(tissue-engineering)"为检索词,应用计算机检索维普数据库,Pubmed数据库,Elsevier数据库相关文章。纳入与视神经损伤后基因治疗修复密切相关的文献,排除重复性研究。结果与结论:共检索到268篇文献,排除无关重复的文献,保留29篇文献进行综述。目前视神经损伤后,越来越多的研究采用非病毒及病毒载体从基因角度感染视网膜节细胞,其中病毒载体包括慢病毒载体,腺病毒载体,腺相关病毒载体,当前大部分研究是采用重组的腺相关病毒载体。通过这些基因载体工具的应用,能增强视网膜节细胞的存活,促进受损视神经轴突的再生。BACKGROUND：Current treatments for optic nerve disease include cell transplantation,gene therapy,medication and operation intervention.Moreover,gene therapy has aroused increasing attention and may become an alternative to some optic nerve diseases.OBJECTIVE：To summarize gene therapy for optic nerve injury from aspects of gene vector,neurotrophic factor,and tissue engineering.METHODS：A computer-based online search of VIP,Pubmed and Elsevier databases was performed for related articles with the Key words ＂optic nerve regeneration,gene therapy,tissue engineering＂ in Chinese and English.Articles related to gene therapy for optic nerve injury were included,and repetitive studies were excluded.RESULTS AND CONCLUSION：A total of 268 articles were collected,and 29 were included.Increasing gene therapies utilized non-virus and viral vector to transfect retinal ganglion cells,including many vector transfection methods,such as non-viral vector transfection,lentiviral vector transfection,adenovirus vector transfection,and adeno-associated virus vector transfection.Recombinant adenovirus vectors have been frequently used.These gene vectors can enhance retinal ganglion cells survival and promote axonal regeneration.

关 键 词：病毒载体 非病毒载体 基因治疗 视神经 修复 组织工程 

分 类 号：R318[医药卫生—生物医学工程]