64排螺旋CT评估碱性成纤维细胞因子基因治疗兔缺血心肌的血管生成  

Application of 64-slice CT imaging in the evaluation of basic fibroblast growth factor on angiogenesis in myocardial ischemia rabbits

作　　者：龙开林[1] 周攀[1] 

机构地区：[1]中南大学湘雅三医院放射科,湖南省长沙市410013

出　　处：《中国组织工程研究与临床康复》2010年第35期6536-6539,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：湖南省卫生厅科研基金资助项目(B2009-065)~~

摘　　要：背景:64排螺旋CT检查以无创性等优点作为冠状动脉狭窄介入治疗前的筛选,在诊断早期冠心病及评价心功能方面具有重要的临床价值。但目前尚无利用64排螺旋CT评估基因治疗效果的研究。目的:应用64排螺旋CT评估碱性成纤维细胞因子基因对兔缺血心肌血管生成的影响。方法:建立新西兰兔急性心肌缺血模型,模型稳定后分别心肌注射200μL携带碱性成纤维细胞因子基因的聚酰胺-胺型纳米载体的磷酸盐缓冲液或磷酸盐缓冲液。4周后,行64排螺旋CT显影观察梗死心肌和左室心肌面积,同时取心肌组织2%TTC染色观察每个层面的梗死心肌和左室心肌面积,并于高倍镜下计数缺血区域新生血管数目。结果与结论:64排螺旋CT显像示治疗组梗死心肌容积百分比显著小于对照组(P<0.05)。治疗组TTC染色梗死心肌容积小于对照组(P<0.05)。治疗组单个高倍视野内的新生血管数显著高于对照组(P<0.05)。结果表明聚酰胺-胺型载体介导的碱性成纤维细胞因子基因能诱导缺血心肌血管生成,64排螺旋CT可以用来评估心肌缺血基因治疗效果。BACKGROUND： 64-slice spiral CT provides noninvasive approach that can potentially expand the utility of coronary angiography. However, there are few reports concerning 64-slice spiral CT in evaluating therapeutic effect of gene therapy. OBJECTIVE： To evaluate the effect of basic fibroblast growth factor （bFGF） on angiogenesis in myocardial ischemia using 64-slice spiral CT. METHODS： New Zealand rabbits with acute myocardial ischemia were randomly assigned to the bFGF group and control group. Totally 200 μL buffer phosphate containing polyamidoamine carrying bFGF cDNA nano-carriers or buffer phosphate only was injected into ischemia myocardia. Four weeks after injection, 64-slice spiral CT imaging and immunohistochemistry were applied to examine the region of ischemia myocardial and increased density of capillaries. RESULTS AND CONCLUSION： 64-slice spiral CT imaging demonstrated that, the percentage of ischemia myocardial region in the treatment group was obviously smaller than that of the control group （P 0.05）. Furthermore, density of capillaries and arteries were increased in the myocardial section of the treatment group （P 0.05）. The findings indicated that, gene therapy with PAMAM-bFGF induces arteriogenesis in the region of myocardial ischemia. 64-slice spiral CT can evaluate therapeutic efficacy of gene therapy for myocardial ischemia.

关 键 词：碱性成纤维细胞因子 64排螺旋CT 聚酰胺-胺型纳米载体 心肌缺血 基因治疗 

分 类 号：R318[医药卫生—生物医学工程]