双光子成像观测拟南芥细胞骨架及根尖生长过程  

Observation on Cytoskeleton and Root Tip Growth of Arabidopsis Thaliana by Two-Photon Laser Scanning Microscopy

作　　者：王鹏飞[1] 林丹樱[1] 马万云[1] 

机构地区：[1]清华大学物理系,原子分子与纳米科学教育部重点实验室,北京100084

出　　处：《光谱学与光谱分析》2010年第10期2593-2596,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(90919012;10874099);教育部博士点基金项目(20090002110065)资助

摘　　要：细胞骨架在植物细胞及其运动、发育变化等过程中起着重要作用。本文利用MS培养基在无菌条件下培养转染GFP(绿色荧光蛋白)的拟南芥,使其经过从种子萌发、植株生长直至开花结果的完整生命周期;在此过程中,利用适合对较大较厚样品进行活体四维成像的双光子激光扫描显微术,观测活的拟南芥种子、根尖、导管和根毛等器官内的细胞骨架形态,以及拟南芥根尖生长发育的动态过程,量化计算出根尖的生长速度。用低浓度(10-10mol.L-1)吲哚-3-乙酸刺激拟南芥,观测到根尖生长速度比正常情况下提高约3.8倍。Cytoskeleton plays an important role in plant cells and their movements,developments,and so on.With MS culture medium,the present paper cultured GFP-fused Arabidopsis thaliana in asepsis condition,which completed its whole lifecycle in the lab,starting from germination,going through plant growth,blossom,and ending at fructification.During this process,the authors used two-photon laser scanning microscopy（TPLSM） which was suitable for 4D observation on large,thick,live samples to observe cytoskeleton in seed,root tip,vessel and root hair of the Arabidopsis thaliana.Dynamic root tip growth as well as the growing speed was also observed.Applying IAA to the Arabidopsis thaliana,the authors found that the growing speed of root tip was enhanced by about 3.8 times.

关 键 词：双光子成像 拟南芥 GFP转染 细胞骨架 

分 类 号：O439[机械工程—光学工程]