白虎汤中钙元素的化学形态分析  

Study on the Speciation of Calcium in Baihu Decoction

作　　者：吕培[1] 李祥[1] 陈建伟[1] 

机构地区：[1]南京中医药大学药学院,江苏南京210046

出　　处：《光谱学与光谱分析》2010年第10期2824-2826,共3页Spectroscopy and Spectral Analysis

基　　金：国家科技部"十一五"支撑计划项目(2006BAI09B06-04);江苏省中药炮制重点实验室课题项目(ZYPZ005)资助

摘　　要：应用螯合树脂、阳离子交换树脂以及有机溶剂萃取等分离方法对白虎汤水煎液中Ca的不同形态进行富集分离,采用电感耦合等离子体原子发射光谱法(ICP-AES)对不同形态的Ca含量进行测定,钙元素的检出限为0.038μg.mL-1,RSD为1.5%,离子态和无机态的回收率分别为95.4%和91.8%。白虎汤水煎液中可溶性Ca的离子态比例为83.25%,有机结合态比例为23.79%,其中含有少量的稳定结合态。白虎汤水煎液中溶出的Ca是以离子态为主,多种形态共同存在的复杂水溶平衡体系。A method for speciation analysis of calcium in Baihu decoction was presented.The six different forms of Ca in the filtrate including free ions,non-free ions,exchangeable,non-exchangeable,organic and inorganic were separated by using ion-exchange resin（IER）,chelating resin and organic solvent extraction.Various forms of Ca were determined by inductively coupled plasma atomic emission spectrometry（ICP-AES）.The recovery for the method is 90.0%-110.0%.The result showed that Baihu decoction extract was a kind of complex solution,in which 83.25% of Ca existed in free ions form and 23.79% of Ca existed in organic form in the soluble filtrate.

关 键 词：白虎汤 石膏 钙 化学形态 ICP-AES 

分 类 号：O657.3[理学—分析化学] R284[理学—化学]