逍遥散对模拟应激体外培养大鼠海马神经细胞内Ca^2＋水平的影响  

Mechanism of Xiao Yao San influences concentration of Ca^2＋ intracellular in cultured rat hippocampal neurons in the state of chronic stress

作　　者：敖海清[1] 孙琪[1] 徐志伟[1] 王文竹[1] 富文俊[1] 

机构地区：[1]广州中医药大学基础医学院,广州510006

出　　处：《中华行为医学与脑科学杂志》2010年第9期820-822,共3页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然科学基金（30801525）;广东省中医药强省基金（2007054）;广州中医药大学创新基金（K0070010）

摘　　要：目的 观测逍遥散对模拟应激体外培养大鼠海马神经细胞内Ca^2＋水平的影响.方法 体外培养海马神经细胞为研究对象,分为1.空白对照组、2.正常血清对照组、3.正常血清＋Glu组、4.模型血清＋Glu组、5.模型血清＋Glu＋MK801组、6.逍遥散治疗血清＋Glu组、7.逍遥散治疗血清＋Glu＋MK801组,以MK-801为工具药,通过激光共聚焦显微镜技术对模拟慢性应激微环境下以及逍遥散治疗血清干预后大鼠海马神经细胞内Ca^2＋浓度进行检测.结果 与第1组（779.97±36.81）相比较,第2组（1092.38±36.41）、第3组（1472.49±76.19）、第4组（1509.52±104.69）、第5组（1186.97±41.92）组荧光强度均明显升高,差异有显著性（P＜0.01）,第6组（908.74±40.24）也有所升高（P＜0.05）;与第2组相比较,第3、4、6组荧光强度均明显升高,差异有显著性（P＜0.01）,第7组（721.99±60.33）则明显降低,也差异有显著性（P＜0.01）;与第4组相比较,第6,7组荧光强度均明显降低,差异有显著性（P＜0.01）,第5组也有所降低（P＜0.05）;与第6组相比较,第5组荧光强度均明显升高,第7组明显降低,均差异有显著性（P＜0.01）.结论 逍遥散可能通过包括谷氨酸-N-甲基,D-天门冬氨酸受体-钙离子（Glu-NR-Ca^2＋）在内的多种细胞信号途径维持胞内钙稳态从而保护神经元免受兴奋性神经毒性的作用.Objective To investigate the effects of Xiao Yao San on intracellular Ca2 ＋ concentration in cultured rat hippocampal neurons in the state of chronic stress and study the mechanism of chronic stress injuring and XiaoYao San protecting. Methods MK-801 acts as tool,cultured rat hippocampal neurons were divided into seven groups, those were group 1 （control）, group 2 （normal serum）, group 3 （normal serum ＋ Glu）, group 4 （model serum ＋ Glu）, group 5 （model serum ＋ Glu ＋ MK-801）, group 6 （Xiaoyaosan ＋ Glu）, group 7 （Xiaoyaosan ＋ Glu ＋ MK-801）. to detect intracellular Ca^2＋ concentration in cultured hippocampal neurons in the simulated micro - environment of chronic stress and after intervention with the serum treated with Xiao Yao San by confocal laser microscope at the same period of time. Results Compared with group 1 （779.97 ± 36.81）, concentration of Ca^2＋ intracellular of group 2 （1092.38 ± 36.41）, group 3 （1472.49 ± 76. 19）, group 4 （1509.52 ±104.69） and group 5 （1186.97 ±41.92） all increased significantly （P〈0.01） ,group 6 （908.74 ±40.24） increased too （P 〈 0.05）, compared with group 2, concentration of Ca2 ＋ intracellular of group 3,4 and 5 all increased significantly （P 〈 0.01）, but group 7 （721.99 ± 60.33） decreased significantly （P 〈 0.01）. Compared with group 4, concentration of Ca^2＋ intracellular of group 6 and 7 decreased significantly （P〈 0.01）, group 5 decreased too （P 〈 0.05）, compared with group 6, concentration of Ca2 ＋ intracellular of group 5 increased significantly （P 〈 0.01）,when group 7 decreased significantly （P〈0.01）. Conclusion Serum of chronic stress treated with Xiao Yao San has the effect of inhibiting Ca^2＋ overload in hippocampal neurons,it may work through a variety of signaling pathways including Glu-NR-Ca^2＋ to maintain the steady-state of Ca^2＋ concentration in hippocampal neurons, and then to protect neurons from the neurotoxic effects of e

关 键 词：逍遥散 慢性应激 大鼠 海马神经细胞 钙离子 

分 类 号：R285.5[医药卫生—中药学]