阿霉素对老年大鼠血浆及皮肤创面TNF_α含量的影响  

The effect of adriamycin on TNF α level in plasma and healing wound in aging wounded rats

作　　者：李萍[1] 盛巡[1] 徐宝婴[1] 杨丽彩[1] 丁瑞[1] 文红林 黄启福[2] 

机构地区：[1]北京市中医研究所 [2]北京中医药大学

出　　处：《中国病理生理杂志》1999年第7期604-606,共3页Chinese Journal of Pathophysiology

基　　金：北京市自然科学基金

摘　　要：目的：ＴＮＦα参与伤口愈合的生物学调节的多个环节，本实验以老年大鼠为模型，观察阿霉素导致的慢性伤口愈合过程中血浆、组织ＴＮＦα的变化。方法：２４月龄大鼠在实验前４ｄ尾静脉注射阿霉素８ｍｇ／ｋｇ体重，然后背部切除直径为１５ｃｍ的皮肤，观察伤口区和血浆中ＴＮＦα在伤口愈合过程中的变化。结果：阿霉素可导致伤口愈合强度由对照组的（８６８±１５６）ｋｇ／ｃｍ２降至（６３６±１３６）ｋｇ／ｃｍ２（Ｐ＜００５），表现为伤口愈合障碍。阿霉素攻击后血液白细胞在第７ｄ降至最低，第１２ｄ回升至最高。此时阿霉素攻击第７ｄ血浆ＴＮＦα由实验前的（０７１±０６８）ｎｇ／Ｌ降至（０６５±０１０）ｎｇ／Ｌ，第１１ｄ为（０６８±０１５）ｎｇ／Ｌ，与实验前相比Ｐ＜００５。而生理盐水对照组则由实验前的（０７４±０１０）ｎｇ／Ｌ升至第１１ｄ（０８５±０１２）ｎｇ／Ｌ（Ｐ＜００５），与白细胞的变化趋势相近。阿霉素攻击后组织ＴＮＦα在第７ｄ为（４６２±１８６）ｎｇ／ｇ，与对照组（９９５±２．１６）ｎｇ／ｇ相比Ｐ＜００５，实验第１１ｄ两组ＴＮＦα都有升高，尤其阿霉素组升高至（１０６±１１２）ｎｇ／ｇ，比实?AIM:Changes of TNF α in plasma and wound tissue were studied on the model of wounded aging rats.METHODS:24 months Wistar rats were divided into two groups, NS and ADM. AΦ 1.5 cm skin was cut on the back after 4 days of injection of NS and ADM 8 mg/kg body weight.RESULTS:Adrinmycin injection decreased the wound breaking strength of healing wound from (8 68±1 56) kg/cm 2 of NS group to (6 36±1 36) kg/cm 2 of adriamycin group. It also depressed WBC count which was most marked by day 6 later and rebound to the level above the controls by day 12. The level of TNF α in plasma of NS group increased from (0 74±0 10) ng/L prior to experiment to 0 85±0.12 ng/L 7 days after wounding ( P <0.05). The level of TNF α in plasma of adriamycin group decreased from (0 71±0 68) ng/L prior to experiment to (0 65±0 10) ng/L 3 days after wounding and to (0 68±0 15) ng/L 7 days after ( P <0 05). The level of TNF α in healed tissue of adriamycin injection group at wound day 3 is lower than that of NS group, they are respectively (4 62±1 86) ng/g and (9 95±2 16) ng/g ( P <0.05). At wound day 7, the values of both groups increased, especially that of adriamycin group, it reached (10 6±1 12) ng/g, compared with the value of wound day 3 in adriamycin group ( P <0 01).CONCLUSION:The changes of TNF α in plasma and healed wound tissue is related to the WBC count, which is the resource of TNF α. The decrease of TNF α leads to wound healing impairment.

关 键 词：伤口愈合 肿瘤坏死因子 阿霉素 老年 大鼠 

分 类 号：R641.02[医药卫生—外科学] R979.14[医药卫生—临床医学]