红景天甙对缺氧状态下兔肺动脉平滑肌细胞增殖和c-fos、c-myc原癌基因表达的影响  

Effects of salidroside on the proliferation and c- fos , c- myc oncogene expression of rabbit pulmonary artery smooth muscle cells under hypoxia

作　　者：赵贺玲[1,2] 林树新[1,2] 贾斌 张莉莉[1,2] 张世范 

机构地区：[1]第四军医大学病生教研室 [2]兰州军区总医院胸外科

出　　处：《中国病理生理杂志》1999年第7期656-659,共4页Chinese Journal of Pathophysiology

摘　　要：目的：研究红景天甙（ｓａｌｉｄｒｏｓｉｄｅ，Ｓａｌ）对缺氧（２％～３％）状态下兔肺动脉平滑肌细胞（ＰＡＳＭＣ）增殖、ＤＮＡ合成、细胞周期和ｃ－ｆｏｓ，ｃ－ｍｙｃ原癌基因表达的影响。方法：应用细胞培养、四唑盐比色试验（ＭＴＴ）、［３Ｈ］－胸腺嘧啶核苷（［３Ｈ］－ＴｄＲ）掺入、细胞周期测定和斑点杂交的方法。结果：发现缺氧２４ｈ可直接刺激ＰＡＳＭＣ，使ＭＴＴ之ＯＤ值增加９５％（Ｐ＜００５），［３Ｈ］－ＴｄＲ的掺入量增加１４０％（Ｐ＜０．０１）；在缺氧的ＰＡＳＭＣ培养液中加入Ｓａｌ（１２００μｇ／ｍＬ）可抑制缺氧的这种促增殖作用，ＰＡＳＭＣ的ＭＴＴ之ＯＤ值与［３Ｈ］－ＴｄＲ掺入量与缺氧组相比显著下降（Ｐ＜０．０５）；流式细胞分析显示缺氧２４ｈＰＡＳＭＣＧ２／Ｍ期细胞比例比常氧组明显增多（Ｐ＜０．０１），Ｇ０／Ｇ１期细胞比例明显减少（Ｐ＜０．０５），与缺氧组相比Ｓａｌ可增多ＰＡＳＭＣＧ０／Ｇ１期细胞比例，减少Ｇ２／Ｍ期细胞比例（Ｐ＜０．０５）；缺氧可促进ｃ－ｆｏｓ，ｃ－ｍｙｃ原癌基因表达，该效应可被Ｓａｌ所抑制。结论：红景天甙可抑制缺氧所致的ＰＡＳＭＣ增殖、ＤＮＡ合成、进入Ｇ２／Ｍ期的细胞比例和ｃ－ｆｏｓ，ｃ－ｍｙ?AIM: To investigate the effects of salidroside on the proliferation, DNA synthesis, cell cycle and c- fos , c- myc oncogene expression of rabbit PASMC(pulmonary artery smooth muscle cell) under hypoxia. METHODS:Techniques of cell culture, MTT test, -TdR incorporation, flowcytometric DNA analysis and Dot blot were used. RESULTS: OD value of MTT and -TdR incorporation to PASMC increased significantly by 95% ( P<0.05 ) and 140% ( P<0 01 ) respectively after 24 h hypoxia, and salidroside(1 200 μg/mL) could inhibit these effects induced by hypoxia( P <0 05); Flowcytometric DNA analysis revealed that hypoxia induced significant enhancement of G 2/M phase of PASMC ( P<0 01 ) and decreased G 0/G 1 phase of PASMC( P <0 05); While salidroside could reverse these effects ( P <0 05); hypoxia stimulated c- fos , c- myc oncogene expression of PASMC, and the effect could be inhibited by salidroside. CONCLUSION:Salidroside inhibited the proliferation, the cell proportion of G 2/M phase, DNA synthesis and c- fos , c- myc oncogene expression of PASMC induced by hypoxia.

关 键 词：缺氧 肺动脉高压 C-FOS基因 红景天甙 C-MYC基因 

分 类 号：R594.306[医药卫生—内科学] R543.202[医药卫生—临床医学]